Our objective was to study the incidence of sperm-tail phosphotyrosine immunoreactivity in normozoospermic and asthenozoospermic human sperm samples, its association with sperm motion parameters, particularly hyperactivated motility, and its potential involvement in the pathogenesis of asthenozoospermia. The work was conducted as a prospective experimental study in the Sperm Biology and Androlo...

background: the health effect of rapidly increasing everyday exposure of humans to radiofrequency radiation is a major global concern. mobile phone jammers prevent the mobile phones from receiving signals from base stations by interfering with authorized mobile carriers’ services. in spite of the fact that mobile jammer use is illegal, they are occasionally used in offices, shrines, conference ...

BACKGROUND Sperm DNA integrity has been used as a new marker of sperm quality in the prediction of pregnancy. Nevertheless, no previous study has been performed by analysing the same samples that were employed in assisted reproduction. The main objective of this work was to correlate sperm chromatin dispersion (SCD), measured by the SCD test, with semen parameters and pregnancy outcome in intra...

BACKGROUND Protein tyrosine phosphorylation (TP) of human sperm is related to sperm capacitation and zona pellucida (ZP) binding. The aim of this study was to determine whether the TP of capacitated sperm is a useful marker for the ability of sperm to bind to the ZP and undergo the ZP-induced acrosome reaction (AR). METHODS Semen samples were obtained from 115 subfertile men with sperm count ...

introduction: cryopreservation of single human spermatozoa is a new technique designed to improve the management of male infertility, it was first introduced in 1997 and was reported by others latter. the aim of this research was to evaluate the efficiency of this method in preserving either ejaculated or surgically retrieved spermatozoae. materials and methods: spermatozoae were obtained from ...

There is concern that intracytoplasmic sperm injection (ICSI) may lead to offspring with a high frequency of chromosomal abnormalities. Accordingly, we studied spermatozoa sampled from eight infertile men with oligoasthenoteratozoospermia (OAT) by multi-colour fluorescence in-situ hybridization (FISH), using DNA probes for chromosomes 13, 18, 21, X and Y. Results were compared with those of spe...

BACKGROUND The association between infertility and sperm disomy is well documented. Results vary but most report that men with severely compromised semen parameters have a significantly elevated proportion of disomic sperm. The relationship between individual semen parameters and segregation of specific chromosome pairs is however less well reported as is the variation of disomy levels in indiv...

Isodicentric chromosomes are the most commonly reported aberrations of the human Y chromosome. We characterized the isodicentric Y chromosome from an azoospermic male by fluorescence in situ hybridization (FISH) and PCR. The patient was a 33-year-old man who visited our hospital for evaluation of infertility. He expressed male external genitalia and showed normal testosterone levels. However, s...

Background: Parental anxiety while waiting for the results of amniocentesis has been investigated by many authors. It seems that the implementation of faster techniques such as fluorescence in-situ hybridization (FISH) will have some benefits in reducing this anxiety. Besides the patients' attitudes to choosing this method, gynecologists who are the persons responsible for treatment, must feel ...

Teratospermia (>60% of morphologically abnormal spermatozoa) is well documented in felids. Even morphologically normal spermatozoa from teratospermic ejaculates have reduced ability to undergo tyrosine phosphorylation, acrosome react, and bind and penetrate oocytes compared with normospermic (<40% abnormal spermatozoa) counterparts. However, it is unknown whether fertilization deficiencies orig...