Changes to larval fish assemblages may have far reaching ecological impacts. Correlations between habitat modification, contamination and marine larval fish communities have rarely been assessed in situ. We investigated links between the large-scale distribution of stressors and larval fish assemblages in estuarine environments. Larval fish communities were sampled using a benthic sled within t...

In northern Tanzania large numbers of small scale miners use mercury in the gold extraction process causing contamination of the environment and risks to human health. Human exposure to Hg was assessed in populations in and around small scale gold mining camps by means of human hair and urine surveys. We also determined Hg concentration in fish in aquatic bodies close to these camps. Urinary Hg...

RNA flow cytometry (FISH-Flow) achieves high-throughput measurement of single-cell gene expression by combining in-situ nucleic acid hybridization with flow cytometry. We tested whether antigen-specific T-cell responses detected by FISH-Flow correlated with latent tuberculosis infection (LTBI), a condition affecting one-third of the world population. Peripheral-blood mononuclear cells from dono...

A comparative analysis was performed to seek large-scale patterns in the relationships between a set of fish species traits (body size, type of environment, trophic level, schooling behaviour, depth range, mean habitat temperature, geographical range, ability to enter brackish waters and capability of migration) and the diversity of their metazoan parasite assemblages among 651 Neotropical fish...

Marine fish abundance and distribution often varies across spatial scales for a variety of reasons, and this variability has significant ecological and management consequences. We quantified the distribution of reef-associated fish species along the southeast United States Atlantic coast using underwater video survey samples (N = 4,855 in 2011-2014) to elucidate variability within species acros...

Artificial reefs are a popular fisheriesmanagement tool, but the effect of these reefs on the abundance of fish in the surrounding pelagic environment is uncertain. Pelagic baited remote underwater video (PBRUV) was used to observe the fish assemblage surrounding an offshore artificial reef (OAR), near Sydney,Australia. PBRUVswere deployed at three distances (30, 100, 500m) from the OAR, and co...

The long-term goals of this program are to (1) instantaneously detect, image and spatially chart fish populations over continental-shelf scales, and (2) continuously monitor the areal densities and behavior of these fish populations over time using a novel audible frequency acoustic system (3005000Hz) referred to as Ocean Acoustic Waveguide Remote Sensing (OAWRS). This new method is being appli...

Fish scales are morphologically diverse among species, within species, and on individuals. Scales of bony fishes are often categorized into three main types: cycloid scales have smooth edges; spinoid scales have spines protruding from the body of the scale; ctenoid scales have interdigitating spines protruding from the posterior margin of the scale. For this study, we used two- and three-dimens...

Fluorescent in-situ hybridization (FISH) and immunohistochemistry (IHC) constitute a pair of complimentary techniques for detecting gene amplification and overexpression, respectively. The advantages of IHC include relatively cheap materials and high sample durability, while FISH is the more accurate and reproducible method. Evaluation of FISH and IHC images is still largely performed manually,...

Gelatin methacryloyl (GelMA) is a versatile biomaterial that has been used in various biomedical fields. Thus far, however, GelMA is mostly obtained from mammalian sources, which are associated with a risk of transmission of diseases, such as mad cow disease, as well as certain religious restrictions. In this study, we synthesized GelMA using fish-derived gelatin by a conventional GelMA synthes...