In industrial fed-batch cultivations it is often necessary to control substrate concentrations at a low level to prevent the production of overflow metabolites and thus optimize the biomass yield. A new method for on-line monitoring and fed-batch control based on fluorescence measurements has been developed. Via instantaneous in situ measurements and multivariate data analysis a chemometric mod...

Abstract: In this paper an optimal control algorithm for E. coli fed-batch fermentation has been developed. A simple material balance model is used to describe the E. coli fermentation process. The optimal feed rate control of a primary metabolite process is studied and a biomass production is used as an example. An optimization of a fed-batch fermentation process is usually done using the calc...

The main target in the production of monoclonal antibodies (MAbs) is reduction of operating costs. One of the pertinent challenges is improving the yield of MAbs through the reduction of secondary metabolic products. Therefore searching for an optimal nutrients supplying strategy becomes mandatory. This study is about the influence of by-products together with the dead cell concentration upon t...

The recombinant product is secreted to the periplasm in many processes where E. coli is used as host. One drawback with secretion is the undesired leakage of the periplasmic products to the medium. The aim of this work was to find strategies to influence the periplasmic retention of recombinant products. We have focused on the role of the specific growth rate, a parameter that is usually contro...

Hydrogen has received considerable attention as a sustainable energy source to the future and biological hydrogen production been gaining consideration potential method. One microorganism that produces is nitrogen-fixing cyanobacterium Cyanothece sp. ATCC 51142, which stands out for its rate of production. However, there are still some challenges understanding improving process by this cyanobac...