The objective in this study was to assess breed effects in fecal prevalence of Escherichia coli O157:H7 in heifers on a development program in Florida and in their steer half siblings in stocker and feedlot phases in Oklahoma. A secondary objective was to characterize fecal shedding of Campylobacter and Salmonella in subsets of the same samples. After weaning, heifers (n = 501; purebreds and F1...

The use of noninvasive genetic sampling to identify the sex of wild animals is an extremely valuable and important tool in molecular ecology and wildlife conservation. Sex determination using the amelogenin gene has been conducted in many species because only a single pair of primers is required to amplify both X- and Y-linked alleles. However, this method has not been used in field research wi...

The present study was undertaken to know the infection status of Cryptosporidium parvum among the residents of Chorwon-gun, Kangwon-do in 1993. Total 461 fecal samples were collected from the inhabitants residing in Chorwon-gun during the period of August 12 to September 14, 1993. Fecal smears were prepared by formalin-ether sedimentation, and examined after modified acid fast staining. Of the ...

Intestinal tuberculosis (ITB) and Crohn's disease (CD) are granulomatous disorders with similar clinical manifestations and pathological features that are often difficult to differentiate. This study evaluated the value of fluorescent quantitative polymerase chain reaction (FQ-PCR) for Mycobacterium tuberculosis (MTB) in fecal samples and biopsy specimens to differentiate ITB from CD. From June...

Real-time PCR was applied to quantify the abundance of human adenoviruses in two southern California urban rivers, the San Gabriel and Los Angeles. A total of 114 river samples from five different locations were collected over a 1-year period and analyzed for human adenoviruses, along with fecal indicator bacteria and coliphages. Adenoviruses were detected by real-time PCR in approximately 16% ...

the presence of Campylobacter dna was studied by means of Pcr in fecal samples from 37 dogs suffering from acute or chronic diarrhea and 50 dogs without clinical signs. in total, 47% of the fecal samples were positive for Campylobacter dna, with C. upsaliensis being the predominant species, followed by C. coli, C. jejuni and C. mucosalis. C. helveticus, C. lari, C. hyointestinalis, C. sputorum,...

Many of Alabama's rivers and streams do not meet the Clean Water Act's "swimmable" goal due to fecal contamination. Catoma Creek in Montgomery County has been included on Alabama's Section 303(d) List due to elevated numbers of fecal coliform bacteria and organic enrichment. Both urban and agricultural land uses exist in the watershed. The objectives of this research were to construct a library...

This research was carried out in order to determine the prevalence of Eimeria spp. Cryptosporidium spp. oocysts and Giardia cysts in calves less than 6 months of age in Van province. For this purpose, fecal samples were obtained from the rectum of 182 calves. Fecal samples (n: 182) were examined with the modified acid-fast technique for Cryptosporidium spp. oocysts. The same samples were examin...

The identification of fecal pollution sources is commonly carried out using DNA-based methods. However, there is evidence that DNA can be associated with dead cells or present as "naked DNA" in the environment. Furthermore, it has been shown that rRNA-targeted reverse transcription-quantitative PCR (RT-qPCR) assays can be more sensitive than rRNA gene-based qPCR assays since metabolically activ...

The objective of this study was the serological, bacteriological and molecular diagnosis, as well as the molecular characterization of Mycobacterium avium subsp. paratuberculosis (Map) in adult cows of five Colombian dairy herds. Serum samples were tested by an indirect absorbed enzyme-linked immunosorbent assay (ELISA-C). All fecal samples were tested by pooled culture. After that, fecal sampl...