conclusions reteplase was expressed in e. coli top 10 after activation of pbad/giiia promoter region by arabinose and optimized. results the obtained recombinant plasmid was sequenced to confirm the presence and correct framing of reteplase gene regarding the expression of reteplase. maximum production of this enzyme was obtained under the following condition: 0.0002% l-arabinose at 37°c for 2 ...

Scientists are nowadays providing great awareness about microarray gene expression dataset. Researches tell that data mining fails to recognize the most important biological associations between genes. Recently biological information mining using clustering techniques were used for the analytical evaluation of gene expression. There are many challenges exist in the existing methods. Optimizatio...

Background: The increase of the protein expression via ribosomal manipulation is one of the suggested cellular mechanisms involved in EnBase fed-batch mode of cultivation. However, this system has not been implemented for cytotoxic proteins.Objectives: Here, the expression pattern of α-Luffin, a ribosome inactivation protein (RIP) with an innate toxicity,...

Background: Leukemia inhibitor factor (LIF) is a very important pleiotropic cytokine which belongs to interleukin-6 (IL-6) family. LIF exerts multiple effects on different types of cells and tissues with numerous regulatory effects in vivo and in vitro. It is a lymphoid factor, which performs a number of activities including cholinergic neuron differentia‌tion, contro...

Background: Mammalian Cell lines are the main expression system for the production of recombinant therapeutic proteins. Optimization of cell culture condition is performed via alteration in different parameter. Cell culture media plays an important role in cell cycle because of compounds such as amino acids, vitamins, inorganic salts, glucose, and serum as a source of growth factors, trace elem...

Optimization of process variables has become very vital in oil extraction processes to obtain maximum yield from oilseeds and nuts. This work focussed on the optimization sandbox seed by mechanical expression. Effects moisture content, roasting temperature, time, expression pressure time was studied using a 5×5 Central Composite Rotatable Design Response Surface Methodology experimental design....

service level agreement, web performance tuning, software performance modeling, response time, queueing, optimization We present a nonlinear integer optimization model for determining the number of machines at each tier in a multi-tier server network. We utilize a result from an open queueing network model on the average response time; the optimization model is to minimize a weighted sum of tot...

Vector quantization is known as the best lossy source coding among the fixed-to-fixed coding methods because of its satisfactory ability of expression. Although it can represent any fixed-to-fixed code and has optimization methods that guarantee local optimality, its encoding and optimization require the computation that grows exponential to the data length. We propose an optimization method fo...