the aim of this study was to produce a recombinant protein consisting of the catalytic and translocation domains of diphtheria toxin for its later application as a vaccine candidate against corynebacterium diphtheria. to achieve this goal, at first, the amino acid sequence of dt386 was used for prediction of t and b cell epitopes using on-line servers. the dt386 coding sequence was synthesized ...

introduction: glycoprotein b (gb) is the major antigen for induction of humoral responses against human cytomegalovirus (hcmv) making it an attractive candidate for immune prophylaxis. in the present study, the humoral immune response of balb/c mice to a truncated hcmv gb protein fused with gfp was evaluated. methods: the truncated gb coding sequence was synthesized and cloned in pegfpn1 eukary...

background: extremely low frequency electromagnetic fields (elf-emf) have been common in daily life all over the world. they have produced by power lines and electrical appliances, but higher levels of them have raised a lot of concerns about their carcinogenesis. both epidemiological and laboratory studies have suggested that emfs might increase cancer incidence, including acute childhood leuk...

allergic rhinitis  (ar) is  an  inflammatory  disorder  of  the  nasal  mucosa  with  high morbidity and prevalence. natural killer (nk) cells might have a role in ar. we aimed to evaluate the changes of the markers and receptors on nk cells in ar patients compared to the non-atopic controls. flow  cytometric  analysis was  used  with  double  staining  of  the  peripheral  blood mononuclear ce...

objective: the aim of the present study was the production of recombinant lentviruses that express mir-16. after transduction, altered expression levels of mirna and its target protein were analyzed. methods: a dna fragment that contained the mir-16 precursor was cloned in a lentiviral plasmid. lentiviral vector particles were produced by transient calcium phosphate co-transfection of 293t cell...

objective: cd44+/cd24-/low breast cancer cells have tumour-initiating properties with stem cell-like features. breast cancer gene 1 (brca1) is a tumour suppressor gene that plays a crucial role in dna repair and maintenance of chromosome stability. the clinicopathological features of breast cancer in brca1 mutation carriers suggest that brca1 may function as a stem-cell regulator. materials and...

introduction: multiple sclerosis is a chronic inflammatory disease of central nervous system. the etiology of ms is slightly known, but genetic and environmental factors are reported. vitamin d regulates gene expression and affects target cell functions. the aim of this study was to investigate the expression variation of il-2 and il-4 genes under vitamin d supplementation in patients with mult...