We established a straightforward experimental system to investigate directly the requirements for nucleocytoplasmic transport in live cells. For this purpose, substrates were created containing nuclear localization signals (NLS) or nuclear export signals (NES) linked to a chimeric protein composed of the glutathione S-transferase (GST) fused to the green fluorescent protein (GFP). The combinati...

The paper evaluates the effect of the FAMEX export promotion program in Tunisia on the export performance of beneficiary firms using firm-level data. Propensity-score matching difference-indifference and weighted least squares estimates suggest that beneficiaries expand at the intensive and the extensive margin (markets and products). However, this expansion is short-lived for total exports. Ou...

Due to the special requirements of distributed systems, it is important that modeling techniques for this kind of systems ooer a stringent module concept. Each module has to support the encapsulation of data structure as well as functionality also at runtime. Modular graph transformation, presented in this contribution, supports these features. Modules are built up of speciications where attrib...

Gene expression in eukaryotes is an essential process that includes transcription, RNA processing, and export. One important player in this interface is the poly(A)(+)-RNA-binding protein Nab2, which regulates the mRNA poly(A)(+)-tail length and export. Here we show that Nab2 has additional roles during mRNA transcription, tRNA metabolism, and ribosomal subunit export. Nab2 is associated with t...

in the present study, competitiveness of iranian pistachio exports and competitor countries in  the world market are studied. pistachio export advantage using indices of revealed comparative advantage (rca) approach and vector error correction model(vecm) based on  agricultural and non-petroleum export commodity basket were investigated separately. the results showed that iran has a comparative...

In over three decades, Bangladesh has witnessed substantial growth in its export of goods and services. Over this period economic growth has accelerated withGDP initially recording an average growth rate of below 3% in the early 1970s and rising to 5% during the 1990s. The trade and industry policies have changed from being highly import substituting and government controlled to become more lib...

It has not been possible to obtain in vitro expression of the positively regulated malE gene encoding the periplasmic maltose-binding protein (MBP) of Escherichia coli. To facilitate in vitro malE expression, we constructed plasmids that place the malE gene under transcriptional control of the lacUV5 promoter-operator. These plasmids could be grouped into three classes, based upon their ability...

Cotranscriptional loading of proteins onto nascent transcripts contributes to the formation of messenger ribonucleoprotein particles (mRNPs) competent for nuclear export. The transcription machinery is believed to play a pivotal role in mRNP assembly, which is at least partially linked to the function of the THO/TREX complex and the mRNA termination/polyadenylation apparatus. Here we demonstrat...

Besides its essential and well established role as a component of the cytoskeleton, actin is also present in the cell nucleus, where it has been linked to many processes that control gene expression. For example, nuclear actin regulates the activity of specific transcription factors, associates with all three RNA polymerases, and is a component of many chromatin remodelling complexes. Despite t...