PURPOSE There is mounting evidence that retinal ganglion cells (RGCs) require a complex milieu of trophic factors to enhance cell survival and axon regeneration after optic nerve injury. The authors' goal was to examine the contribution of components of a combination of hormones, growth factors, steroids, and small molecules to creating a regenerative environment and to determine if any of thes...

The present study was conducted to induce callus from different in vitro grown seedling explants of shoot, root and leaf segments in order to develop shoot buds and rooting from callus. The sterilized seeds were cultured on Murashige and Skoog medium, and then the explants were cultured from seedling and transferred to an MS medium supplemented with different concentrations of BAP, kinetin, NAA...

Fatty acids are considered metabolic intermediaries, although new facts indicate they also work as signaling molecules with different roles in the immune response. Based on that, this study, we investigated anti-inflammatory effects of n-3 polyunsaturated fatty (PUFAs) eicosapentaenoic acid (EPA), docosahexaenoic (DHA), and α-linolenic (LNA) ex vivo bovine endometrial explants. For this, two gr...

Rossi, Noreen F., and William H. Beierwaltes. Nitric oxide modulation of ETB receptor-induced vasopressin release by rat and mouse hypothalamo-neurohypophyseal explants. Am J Physiol Regul Integr Comp Physiol 290: R1208–R1215, 2006. First published December 15, 2005; doi:10.1152/ajpregu.00701.2005.—Endothelin (ET) peptides stimulate vasopressin (AVP) secretion via ETB receptors at hypothalamic ...

Binding of asbestos fibers to the cell surface appears to be important in the initiation of intracellular signaling events as well as in initiation of particle uptake by the cell. We have previously shown that cigarette smoke increases the uptake of asbestos fibers by tracheal epithelial cells in explant culture. Whether smoke acts by increasing surface binding of fibers is not known. In this s...

Abstract Vanda tricolor is an orchid endemic to Mount Merapi in Yogyakarta Indonesia, whose existence threatened due the eruption and exploitation of its original habitat. This conservation effort can be done through vitro culture. Multiplication one culture techniques used for propagation by growing explants on various types multiplication medium. study aims determine effect medium orchids. Th...

Cultured explants of mouse mammary gland were investigated for their capacity to secrete a cathepsin B-like cysteine proteinase. This enzyme had been shown previously to be secreted excessively from cultured explants of human breast tumors and spontaneous mouse mammary carcinomas. We now show that secretion is also observed from cultured explants of mammary gland. Lactating tissue and tissues o...

Elias N, Rafii B, Rahman M, Otulakowski G, Cutz E, O’Brodovich H. The role of -, -, and -ENaC subunits in distal lung epithelial fluid absorption induced by pulmonary edema fluid. Am J Physiol Lung Cell Mol Physiol 293: L537–L545, 2007. First published May 18, 2007; doi:10.1152/ajplung.00373.2006.—Edema fluid (EF) increases epithelial Na transport by rat fetal distal lung epithelia (FDLE) and i...

A method for culturing explants of lamellar hoof was developed to investigate the process of lamellar separation that occurs in laminitis. Explants, consisting of hoof wall, dermal and epidermal lamellae and the adjacent sub-lamellar connective tissue remained intact when cultured in tissue culture medium for 2 days. However, when cultured in the presence of the matrix metalloproteinase (MMP) a...