Malaysian arowana (dragonfish; Scleropages formosus) is an ancient osteoglossid fish from southeast Asia. Due to the high demand of the ornamental fish trade and because of habitat loss, the species is close to extinction. We isolated and characterized 10 polymorphic microsatellites of this species, using 5'-anchored PCR. The number of alleles at the 10 microsatellite loci ranged from 2 to 28, ...

PREMISE OF THE STUDY We developed microsatellite markers for Carpotroche brasiliensis (Flacourtiaceae), a dioecious tree that is used as a food resource by midsize animals of the Brazilian fauna. METHODS AND RESULTS We designed 30 primer pairs using next-generation sequencing and classified 25 pairs as polymorphic. Observed heterozygosity ranged from 0.5 to 1.0, and expected heterozygosity ra...

the japanese and english white quails are widespread strains and belongs to the galliformes order, phasianidae family, coturnix genus  and japonica species. these birds are likely to be well-adapted to the hard conditions and resistance to diseases as it has attained economic importance as an agricultural species. in the current study, the genetic variation of japanese and english white  quail ...

UNLABELLED PREMISE OF THE STUDY We developed 10 new primers for amplifying microsatellite loci for both Leucosceptrum japonicum and L. stellipilum to study the genetic structure of hybrid zones between these two species. • METHODS AND RESULTS We isolated DNA fragments containing microsatellites from an enriched library and designed the 10 primer pairs amplifying the fragments. The number o...

UNLABELLED PREMISE OF THE STUDY Ilex paraguariensis is a native tree species from Brazil, Argentina, and Paraguay that is used in the production of beverages, medicines, and cosmetics. Primers flanking microsatellites were developed to investigate genetic parameters in the species. • METHODS AND RESULTS Using microsatellites cloned from an I. paraguariensis shotgun genomic library, 25 pair...

The single-humped camel (Camelus dromedaries) is bred in the western part of lndia. This breed is famous for high milk yield potential. In the present study, we evaluated the genetic diversity of a population of Malvi camel. Multilocus genotype data on 29 microsatellites were generated on 138 Malvi camel. A total of 240 alleles were observed. The mean observed heterozygosity in camel population...

UNLABELLED PREMISE OF THE STUDY Pinus wangii is an endemic and endangered species in southwestern China, and microsatellite primers were developed to characterize its genetic diversity and population structure. • METHODS AND RESULTS Using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol, nine sets of microsatellite primers were developed in P. wangii. One popula...

PREMISE OF THE STUDY Microsatellite primers were developed to investigate population genetic structure in Lindera glauca (Lauraceae). METHODS AND RESULTS Twenty-five microsatellite primers were developed and optimized for L. glauca using Illumina's Solexa sequencing technology. These novel primers were found to be polymorphic in nine wild L. glauca populations with 81 total alleles confirmed ...

In this study, we isolated 21 novel polymorphic microsatellite DNA loci from the pen shell Atrina pectinata using magnetic-bead hybridization enrichment. The characteristics of these loci were tested using a population of 30 individuals collected from the Penglai coast, Shandong Province. The number of alleles ranged from 2 to 13, and polymorphism information content (PIC) varied from 0.1730 to...

PREMISE OF THE STUDY Microsatellite primers were designed for the submersed aquatic plant Cabomba aquatica s.l. (Cabombaceae) and characterized to estimate genetic diversity parameters. METHODS AND RESULTS Using a selective hybridization method, we designed and tested 30 simple sequence repeat loci using two natural populations of C. aquatica s.l., resulting in 13 amplifiable loci. Twelve loc...