The anaerobic parasitic protozoa Tritrichomonas foetus is found incapable of de novo pyrimidine biosynthesis by its failure to incorporate bicarbonate, aspartate, or orotate into pyrimidine nucleotides or nucleic acids. Uracil phosphoribosyltransferase in the cytoplasm provides the major pyrimidine salvage for the parasite. Exogenous uridine and cytidine are mostly converted to uracil by uridin...

conclusions whenever a cushingoid appearance is accompanied by adrenal insufficiency, the exogenous use of steroids, especially topical creams, should be explored. hypertension may persist even after cessation of use and may result in life threatening complications. case presentation the present report describes the cases of three children and a 25-year-old man who presented with clinical featu...

Cellular retinol-binding protein, type II (CRBP (II] is an abundant protein of the mature enterocytes of the small intestine. It has been shown to direct retinol to an acyl-CoA-independent esterifying activity that utilizes an endogenous acyl donor (Ong, D.E., Kakkad, B., and MacDonald, P.N. (1987) J. Biol. Chem. 262, 2729-2736). Here we report that this activity in intestinal microsomes will c...

The molecular mechanism of the anthracycline-dependent development of cardiotoxicity is still far from being clear. However, it is generally accepted, that mitochondria play a significant role in triggering this organ specific injury. The results presented in this study demonstrate that, in contrast to liver mitochondria, isolated heart mitochondria shuttle single electrons to adriamycin, givin...

The effects of exogenous ascorbic acid (AsA) and reduced glutathione (GSH) on antioxidant enzyme activities [superoxide dismutase (SOD), ascorbate peroxidase (APX), and glutathione reductase (GR)] and the contents of malondialdehyde (MDA) and H2O2, as well as of endogenous AsA and GSH, in the chloroplasts of two rice cultivars, the salt-tolerant cultivar Pokkali and the salt-sensitive cultivar ...

The signalling molecule indole occurs in significant amounts in the mammalian intestinal tract and regulates diverse microbial processes, including bacterial motility, biofilm formation, antibiotic resistance and host cell invasion. In Escherichia coli, the enzyme tryptophanase (TnaA) produces indole from tryptophan, but it is not clear what determines how much indole E. coli can produce and ex...

1. Exogenous microbial beta-1,3-1,4-glucanases and hemicellulases contribute to improving the nutritive value of cereals rich in soluble non-starch polysaccharides for poultry. 2. In general, plant cell wall hydrolases display a modular structure comprising a catalytic module linked to one or more non-catalytic carbohydrate-binding modules (CBMs). Based on primary structure similarity, CBMs hav...

The regulatory role of UDP-glucose in glycogen biogenesis was investigated in fibroblasts containing a point mutation in the UDP-glucose pyrophosphorylase gene and, consequently, chronically low UDP-glucose levels (Qc). Comparisons were made with cells having the intact gene and restored UDP-glucose levels (G3). Glycogen was always very low in Qc cells. [(14)C]Glucose incorporation into glycoge...

to find out the effect of sodium nitroprusside (snp) in reducing oxidative damage induced by salinity stress in barley seedlings, a crd based factorial experiment with three replications was conducted in agronomy laboratory of the faculty of agricultural sciences, university of guilan, in 2012. the experimental design consisted of two snp levels (0.1 and 0.2 mm snp; as nitric oxide (no) donor) ...