Alberti G, Casciola M, Costantino U and Vivani R (1996) Layered and pillared metal(IV) phosphates and phosphonates. Advanced Materials 8(4): 291. Amphlett CB (1964) Inorganic Ion Exchangers. Amsterdam: Elsevier. ClearReld A (ed.) (1982) Inorganic Ion Exchange Materials. Boca Raton, FL: CRC Press. Fritz JS, Gjerde DT and Pohlandt C (1982) Ion Chromatography. Heidelberg: HuK thig. Greig JA (ed.) ...

reversed-phase (ISRP) material. Proteins do not adsorb on the hydrophilic exterior surfaces and do not penetrate into the hydrophobic interior surfaces, while analytes can reach the interior surfaces and be separated. (Reproduced with permission from Perry JA (1991) The internal surface reversed phase. Concept and applications. Journal of Liquid Chromatography 13: 1047}1074.) Gelfferich F (1962...

Monoclonal Antibodies (mAbs) have emerged as a unique and exciting group of biological products. They represent a rapidly growing biopharmaceutical market segment with interesting and extremely useful medical applications in the treatment of numerous diseases such as cancer and immunological disorders. The monoclonal antibody purification process that is adopted should be reliable, robust and m...

lipases are diversified enzymes in their properties and substrate specificity, which make them attractive tools for various industrial applications. in this study, an alkaline thermostable lipase producing bacteria were isolated from soil of different regions of isfahan province (iran) and its lipase was purified by ammonium sulfate precipitation and ion exchange chromatography. to select a the...

background: there are varieties of purification techniques for separation of human plasma proteins such as salting out, ion exchange chromatography, and ethanol fractionation. there are limitations for each method, for example in salting out method, the salt has to be removed in an additional step. ion exchange chromatography is difficult for scaling up, and plasma fractionation is a time consu...

The methods employed previously in this laboratory for the isolation of oxytocin and vasopressin depend upon the use of several procedures to arrive at a highly purified sample. From the acetone powder of the posterior pituitary gland, the extraction and fractionation method of Kamm and coworkers (1) leads to a fraction rich in oxytocin and one rich in vasopressin, each at a stage of intermedia...

Abbreviations: FVIII/vWF: Complex of Factor VIII and von Willebrand Factor; MW: Molecular Weight; NDF: Number of Degree of Freedom; S/D: Solvent/Detergent; LEV/LEVs: Virus (es) Enveloped by Lipid Membrane; NLEV/NLEVs: Non-enveloped Virus (es); IFA method: Method of Immune-fermental Analysis; FITC: Fluorescein Isothiocyanate; RT-PCR method: Real-time PCR Method; TCID50: 50% Tissue Culture Infect...

The single biggest problem with solution-phase H/D exchange as a mass spectrometric probe of surface exposure in a protein (or protein complex) is back-exchange of H for D after the initial H/D exchange has been quenched. Back-exchange results in loss of pertinent data and also greatly hampers data analysis. Previously, very fast, cold (0-4 degrees C) HPLC was performed to help reduce back-exch...