BACKGROUND Pectin-rich wastes, such as citrus pulp and sugar beet pulp, are produced in considerable amounts by the juice and sugar industry and could be used as raw materials for biorefineries. One possible process in such biorefineries is the hydrolysis of these wastes and the subsequent production of ethanol. However, the ethanol-producing organism of choice, Saccharomyces cerevisiae, is not...

BACKGROUND Sustainable and economically viable manufacturing of bioethanol from lignocellulose raw material is dependent on the availability of a robust ethanol producing microorganism, able to ferment all sugars present in the feedstock, including the pentose sugars L-arabinose and D-xylose. Saccharomyces cerevisiae is a robust ethanol producer, but needs to be engineered to achieve pentose su...

The heterologous expression of a highly functional xylose isomerase pathway in Saccharomyces cerevisiae would have significant advantages for ethanol yield, since the pathway bypasses cofactor requirements found in the traditionally used oxidoreductase pathways. However, nearly all reported xylose isomerase-based pathways in S. cerevisiae suffer from poor ethanol productivity, low xylose consum...

In this study, the primary paper-mill sludge characterized as containing 51% glucan was used to optimize enzymatic saccharification process for production of bioethanol using a Box–Behnken design (BBD). Polyethylene glycol 4000 (PEG-4000) surfactant-assisted dried (DPS) showed 12.8% improvement in efficiency. There statistically significant effect solid enzyme loading and time on DPS at 95% con...

For ethanol production from lignocellulose, the fermentation of xylose is an economic necessity. Saccharomyces cerevisiae has been metabolically engineered with a xylose-utilizing pathway. However, the high ethanol yield and productivity seen with glucose have not yet been achieved. To quantitatively analyze metabolic fluxes in recombinant S. cerevisiae during metabolism of xylose-glucose mixtu...

Metabolic engineering is a powerful method to improve, redirect, or generate new metabolic reactions or whole pathways in microorganisms. Here we describe the engineering of a Saccharomyces cerevisiae strain able to utilize the pentose sugar L-arabinose for growth and to ferment it to ethanol. Expanding the substrate fermentation range of S. cerevisiae to include pentoses is important for the u...

Efficient and cost-effective fuel ethanol production from lignocellulosic materials requires simultaneous cofermentation of all hydrolyzed sugars, mainly including D-glucose, D-xylose, and L-arabinose. Saccharomyces cerevisiae is a traditional D-glucose fermenting strain and could utilize D-xylose and L-arabinose after introducing the initial metabolic pathways. The efficiency and simultaneous ...

Bioethanol was produced from the three different agro-industrial biomass residues, i.e., sugarcane bagasse (SB), rice husk (RH) and corn cob (CC)) at 35°C, 120hr with 90g of each substrate. 2% H2SO4 used for hydrolysis samples while 3g yeast (saccharomyces cerevisiae) fermentation. Simple distillation fermented broth. The concentration reducing sugar ethanol, quantity bioethanol as well physica...

The present work consists of producing bioethanol from potato (Solanum tuberosum L.) peels using Saccharomyces cerevisiae. different physicochemical and biochemical analyses showed that are rich in nutritional elements make them favorable to alcohol fermentation. total soluble sugars content, the pH value ethanol content were evaluated. results indicated limited production. addition yeast extra...

Brazil is a major powerhouse in the production of sugarcane. Consequently, several supply chains use it as raw material, such food sector, mainly sugar and beverages, cachaça, biofuels with ethanol, an important product for Brazilian economy. The cachaça ethanol share stage known fermentation, fundamental process that defines quality yield alcoholic fermentation product, which achieved using Sa...