In intact neonatal piglets, two strains of enterotoxigenic Escherichia coli (ETEC), which could adhere to epithelial cells and thus colonize the small intestine, attracted greater numbers of neutrophils into the lumen and wall of the intestine than did a nonenteropathogenic strain of E. coli. Ligated loops of small intestine in 8-week-old pigs were used in attempts to identify the attributes of...

Background: During the last two decades, significant advances have been made in the fields of lactococcal genetics and protein expression. Lactococcus lactis (L. lactis) is an effective vector for protein expression and can be used as an antigen delivery system. Hence, L. lactis is an ideal candidate for mucosal immunotherapy. Profilin (Che a 2), the major allergen in Chenopodium album, is one ...

Background.  Enterotoxigenic Escherichia coli (ETEC) and non-O157 Shiga toxin-producing E. coli (STEC) are not detected by conventional culture methods. The prevalence of ETEC infections in the United States is unknown, and recognized cases are primarily associated with foreign travel. Gaps remain in our understanding of STEC epidemiology. Methods.  Two sentinel surveillance sites were enrolled...

Enterotoxigenic Escherichia coli (ETEC) strains were isolated from travelers or military personnel who developed diarrhea after visiting Nepal or who were deployed to Thailand, Indonesia, or the Philippines. ETEC isolates were examined for colonization factor antigen (CFA). CFAs were identified on 59% (40 of 68) of the isolates examined. The lack of a detectable CFA on 41% (28 of 68) of the iso...

Enterotoxigenic Escherichia coli (ETEC)-induced diarrhoea is the second most commoncause of death in children in the developing countries. Heat labile toxin (LT) is responsible forETEC-induced diarrhoea. In the present study, a novel live ETEC vaccine based on subunitB of LT (LTB) expression in attenuated PhoPc Salmonella strain was developed. Herein, weaimed to compare the in-vitro activity of...

Enterotoxigenic Escherichia coli (ETEC)-induced diarrhoea is the second most commoncause of death in children in the developing countries. Heat labile toxin (LT) is responsible forETEC-induced diarrhoea. In the present study, a novel live ETEC vaccine based on subunitB of LT (LTB) expression in attenuated PhoPc Salmonella strain was developed. Herein, weaimed to compare the in-vitro activity of...

Enterotoxigenic Escherichia coli (ETEC), defined by their elaboration of heat-labile (LT) and/or heat-stable (ST) enterotoxins, are a common cause of diarrheal illness in developing countries. Efficient delivery of these toxins requires ETEC to engage target host enterocytes. This engagement is accomplished using a variety of pathovar-specific and conserved E. coli adhesin molecules as well as ...

A total of 373 isolates of Escherichia coli were obtained from one or more calves with diarrhea in 155 herds during the 1974 calving season in Montana. Sixty-seven (18 percent) of the isolates representing 59 of the 155 herds were found to be enterotoxigenic as indicated by their ability to cause distention of the calf ligated intestinal segment. The 67 isolates of enterotoxigenic E. coli (ETEC...

Enterotoxigenic Escherichia coli (ETEC) is an important pathogen responsible for secretory diarrhoea. The production of heat labile enterotoxin (LT), by ETEC, is largely responsible for the pathogenesis of diarrhoea. In the present study we investigated the effect of stress factors such as temperature, pH, osmotic stress and nutritional limitation on the production of LT by ETEC using in-house ...

Background and Purpose: Diarrhoeagenic E. coli (DEC) is one of the germs responsible for childhood diarrhea in developing countries. This study aims at determining prevalence five main pathotypes DEC isolated from faeces children under years old with or not, living city Koula-Moutou. Methodology: Isolates were phenotypically screened on chromIDTM agar molecularly by multiplex PCR to detect pres...