نتایج جستجو برای: est ssr markers

تعداد نتایج: 251106  

2014
Zan Wang Guohui Yu Binbin Shi Xuemin Wang Haiping Qiang Hongwen Gao

Sufficient codominant genetic markers are needed for various genetic investigations in alfalfa since the species is an outcrossing autotetraploid. With the newly developed next generation sequencing technology, a large amount of transcribed sequences of alfalfa have been generated and are available for identifying SSR markers by data mining. A total of 54,278 alfalfa non-redundant unigenes were...

2013
Claid Mujaju Jasna Sehic Hilde Nybom

Fifteen expressed sequence tag (EST)-derived simple sequence repeats (EST-SSRs) were used to investigate genetic diversity in 139 plants obtained from seeds of 35 watermelon accessions collected from all the geographical provinces of Zimbabwe. In addition, 15 plants representing three commercial varieties developed in the United States (USA) were analyzed for comparison. A total of 65 alleles w...

2013
Kajal Kumari Mehanathan Muthamilarasan Gopal Misra Sarika Gupta Alagesan Subramanian Swarup Kumar Parida Debasis Chattopadhyay Manoj Prasad

Foxtail millet (Setariaitalica L.) is a tractable experimental model crop for studying functional genomics of millets and bioenergy grasses. But the limited availability of genomic resources, particularly expressed sequence-based genic markers is significantly impeding its genetic improvement. Considering this, we attempted to develop EST-derived-SSR (eSSR) markers and utilize them in germplasm...

2014
Walid El-Rodeny Mitsuhiro Kimura Hideki Hirakawa Attia Sabah Kenta Shirasawa Shusei Sato Satoshi Tabata Shigemi Sasamoto Akiko Watanabe Kumiko Kawashima Midori Kato Tsuyuko Wada Hisano Tsuruoka Chika Takahashi Chiharu Minami Keiko Nanri Shinobu Nakayama Mitsuyo Kohara Manabu Yamada Yoshie Kishida Tsunakazu Fujishiro Sachiko Isobe

To develop a high density linkage map in faba bean, a total of 1,363 FBES (Faba bean expressed sequence tag [EST]-derived simple sequence repeat [SSR]) markers were designed based on 5,090 non-redundant ESTs developed in this study. A total of 109 plants of a 'Nubaria 2' × 'Misr 3' F2 mapping population were used for map construction. Because the parents were not pure homozygous lines, the 109 ...

Journal: :PloS one 2015
Jihong Hu Songtao Gui Zhixuan Zhu Xiaolei Wang Weidong Ke Yi Ding

Genomic resources such as single nucleotide polymorphism (SNPs), insertions and deletions (InDels) and SSRs (simple sequence repeats) are essential for crop improvement and better utilization in genetic breeding. However, the resources for the sacred lotus (Nelumbo nucifera Gaertn.) are still limited. In the present study, to dissect large-scale genomic molecular marker resources for sacred lot...

2016
Naoko Ishikawa Shota Sakaguchi Motomi Ito

PREMISE OF THE STUDY Simple sequence repeat (SSR) markers were developed for Aster savatieri (Asteraceae) and the serpentine variety A. savatieri var. pygmaeus to re-evaluate their taxonomic status. METHODS AND RESULTS Using RNA-Seq data, 22 expressed sequence tag (EST)-SSR markers were developed. Polymorphisms were assessed in A. savatieri and in A. savatieri var. pygmaeus. The average numbe...

2017
Xiaopeng Hao Tao Yang Rong Liu Jinguo Hu Yang Yao Marina Burlyaeva Yan Wang Guixing Ren Hongyan Zhang Dong Wang Jianwu Chang Xuxiao Zong

Grasspea (Lathyrus sativus L., 2n = 14) has great agronomic potential because of its ability to survive under extreme conditions, such as drought and flood. However, this legume is less investigated because of its sparse genomic resources and very slow breeding process. In this study, 570 million quality-filtered and trimmed cDNA sequence reads with total length of over 82 billion bp were obtai...

2013
Wanli Du Jing Wang Liangming Wang Jun Zhang Xinhong Chen Jixin Zhao Qunhui Yang Jun Wu

The aim of this study was to characterize a Triticum aestivum-Psathyrostachys huashanica Keng (2n = 2x = 14, NsNs) disomic addition line 2-1-6-3. Individual line 2-1-6-3 plants were analyzed using cytological, genomic in situ hybridization (GISH), EST-SSR, and EST-STS techniques. The alien addition line 2-1-6-3 was shown to have two P. huashanica chromosomes, with a meiotic configuration of 2n ...

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