Expression of recombinant proteins in Escherichia coli (E. coli) remains the most popular and cost-effective method for producing proteins in basic research and for pharmaceutical applications. Despite accumulating experience and methodologies developed over the years, production of recombinant proteins prone to aggregate in E. coli-based systems poses a major challenge in most research applica...

An outbreak of norovirus (NoV) genotype I and Enterotoxigenic Escherichia coli (ETEC) occurred among US Navy Ship personnel following a visit to Lima, Peru, in June 2008. Visiting a specific area in Lima was significantly associated with illness. While ETEC and NoV are commonly recognized as causative agents of outbreaks, co-circulation of both pathogens has been rarely observed in shipboard ou...

In this study 130 bulk tank milk samples which were delivered to the Pegah Pasturisation Factory inMashhad were collected randomly during the summer months. Samples were firstly enriched in modifiedtrypticase soy broth containing novobiocin, followed by plating onto sorbitol MacConkey agar supplementedwith cefixime and potassium tellurite for isolation of Escherichia coli O157:H7. Consequently ...

Enterotoxigenic Escherichia coli (ETEC) is an important cause of bacterial diarrheal illness, affecting practically every population worldwide, and was estimated to cause 120,800 deaths in 2010. Here, we report the genome sequence of ETEC strain FMU073332, isolated from a 25-month-old girl from Tlaltizapán, Morelos, México.

Enteroaggregative E. coli (EAEC) is an established diarrhoeagenic pathotype. The association with virulence gene content and ability to cause disease has been studied but little is known about the population structure of EAEC and how this pathotype evolved. Analysis by Multi Locus Sequence Typing of 564 EAEC isolates from cases and controls in Bangladesh, Nigeria and the UK spanning the past 29...

Bacteriophage St11Ph5 was isolated from a sewage sample on a particularly phage-resistant uropathogenic Escherichia coli (UPEC) up11 host strain. It appeared to be closely related to bacteriophage G7C, isolated from horse feces; however, it carries a highly divergent host recognition module.

Here, we present the genome sequence of the Escherichia coli bacteriophage UFV-AREG1. This phage was isolated from cowshed wastewater and showed specificity for enterohemorrhagic E. coli O157:H7 (ATCC 43895), E. coli 0111 (CDC O11ab) and E. coli (ATCC 23229).

The transformation frequency of Escherichia coli C-600, continuously cultivated in a chemostat operated at constant dilution rate, increased with increase in agitation rate (impeller speed). Cell counts at each impeller speed remained approximately constant. The phenomenon correlated with changes in mean cell volume associated with the changes in agitation rate.