BACKGROUND AND AIM The localization of hepatitis B virus (HBV) core antigen to the nucleus or cytoplasm of hepatocytes has biological implications for viral packaging and persistence. This study examined the relationship between the localization of hepatitis B virus antigens, histological activity, and viral titer in patients with chronic HBV infection. METHODS Liver biopsies from 110 patient...

The B1C8 monoclonal antibody detects a 180-kDa nuclear matrix-specific protein. The protein is a component of the dense, metabolically active bodies or assemblies revealed by resinless section electron microscopy of the nuclear matrix. These assemblies are scattered through the nuclear interior, enmeshed in a complex network of 11-nm filaments. Resinless section electron microscopy of immunogol...

THE POTENTIAL USE of antibody for diagnostic or therapeutic applications in human cancer may well depend upon the ability to synthesize large quantities of monospecific "anti-tumour" immunoglobulin in vitro. In this respect, the hybridoma technology has produced murine monoclonal antibodies directed against a number of human tumour-associated antigens (Koprowski et al., 1978; Yeh et al., 1979; ...

The purpose of this study was to explore the correlations arterial-phase enhanced CT characteristics hepatocellular carcinoma (HCC) with differentiation degree and expressions proliferating cell nuclear antigen (PCNA) phosphatase tensin homologue (PTEN). Seventy-nine HCC patients who underwent three-phase dynamic contrast-enhanced before surgery were selected observe characteristics. tissue eva...

Amino acid (aa) sequence homologies between viruses and autoimmune nuclear antigens are suggestive of viral involvement in disorders such as systemic lupus erythematosus (SLE) and scleroderma. We analyzed the frequency of exact homologies of greater than or equal to 5 aa between 61 viral proteins (19,827 aa), 8 nuclear antigens (3813 aa), and 41 control proteins (11,743 aa). Both pentamer and h...

Immediate early antigen (IEA) induced in human lung fibroblasts by human cytomegalovirus was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis after immunoprecipitation with IEA-positive human sera. Two polypeptides of 76,000 daltons (76K) and 82K were detectable within 90 min after infection. Polypeptides of similar molecular weight were also found in immunopr...

Immunofluorescent studies were made on chicken kidney cell cultures infected with various temperature-sensitive (ts) mutants of an avian adenovirus, chicken embryo lethal orphan (CELO), which grow well at the nonrestrictive temperature of 31 degrees , but fail to grow at the restrictive temperature of 40 degrees . At 40 degrees some mutants (ts 5, 8, and 10) accumulated viral antigen in the cyt...

  Background and Objectives:  The molecular genetic of the human herpes virus 8 (HHV8) has now been characterized the pathogenesis of kaposi sarcoma (KS). This study attempted to determine the rate of HHU-8 infection in KS in an Iraniann cross sectional study. Method & material: In this cross-sectional study, we used paraffin-embedded specimens of 54 clinically well-characterized kaposi sarcom...

During the last decade, mounting evidence has implicated the human neurotropic virus JC virus in the pathology of colon cancer. However, the mechanisms of JC virus-mediated oncogenesis are still not fully determined. One candidate to mediate these effects is the viral early transcriptional product T-Antigen, which has the ability to inactivate cell cycle regulatory proteins such as p53. In medu...

Background & Aims: The role of P53 and proliferating cell nuclear antigen (PCNA) genes in inflamed and non-inflamed odontogenic cysts is related to cell proliferation but it is unclear. The aim of this study was immunohistochemical evaluation of P53 and PCNA expression in inflamed and non-inflamed dentigerous cysts Methods: In the present study, 23 dentigerous cysts (12 inflamed and 11 non-infl...