The promyelocytic leukemia protein (PML) participates in several cellular functions, including transcriptional regulation, apoptosis and maintenance of genomic stability. A key feature of this protein is its ability to induce the assembly of nuclear compartments termed PML-nuclear bodies (PML-NBs). Here we show that these nuclear structures recruit single-stranded DNA (ssDNA) molecules in respo...

The promyelocytic leukemia protein (PML) is a tumor suppressor critical for formation of nuclear bodies (NBs) performing important functions in transcription, apoptosis, DNA repair and antiviral responses. Earlier studies demonstrated that simian virus 40 (SV40) initiates replication near PML NBs. Here we show that PML knockdown inhibits viral replication in vivo, thus indicating a positive rol...

Our previous studies demonstrated that the promyelocytic leukemia gene, PML, encodes a growth and transformation suppressor. Overexpression of PML inhibits cancer cell growth in vitro and in vivo. In this study, we further explored the possibility of applying PML as a potential agent for developing prostate cancer gene therapy using an adenovirus delivery system. We have constructed and produce...

OBJECTIVE Although the prognosis of natalizumab-associated progressive multifocal leukoencephalopathy (PML) seems to be better than HIV-associated PML, little is known about the long-term functional outcome in multiple sclerosis (MS) patients and the subsequent return of MS disease activity. We evaluated retrospectively 15 patients with natalizumab-associated PML treated at our centre. PATIEN...

Our previous studies demonstrated that PML is a growth suppressor that suppresses oncogenic transformation of NIH/3T3 cells and rat embryo fibroblasts. PML is a nuclear matrix-associated phosphoprotein whose expression is regulated during the cell cycle. Disruption of PML function by t(15;17) in acute promyelocytic leukemia (APL) plays a critical role in leukemogenesis. To further study the rol...

The herpesviruses, like most other DNA viruses, replicate in the host cell nucleus. Subnuclear domains known as promyelocytic leukemia protein nuclear bodies (PML-NBs), or ND10 bodies, have been implicated in restricting early herpesviral gene expression. These viruses have evolved countermeasures to disperse PML-NBs, as shown in cells infected in vitro, but information about the fate of PML-NB...

The Business Process Modelling Notation (BPMN) and the Event-driven Process Chain (EPC) are both frequently used modelling languages to create business process models. While there is a well-defined standard for BPMN, such a standard is missing for EPC. As a standard would be beneficial to improve interoperability among different vendors, this paper aims at providing the means for future EPC sta...

Richard B. Kreider, PhD, MX, EPC, FACSM, FASEP; Anthony L. Almada, MSc; Jose Antonio, PhD, FACSM; Craig Broeder, PhD, FNAASO; Conrad Earnest, PhD, FACSM; Lori Greenwood, PhD, ATC, LAT; Mike Greenwood, PhD, CSCS*D; Thomas Incledon, MS, RD, LD/LN, NSCA-CPT, CSCS, RPT; Douglas S. Kalman MS, RD, FACN; Chad Kerksick, MS, CSCS, ATC, EPC; Susan M. Kleiner, PhD, RD, FACN; Brian Leutholtz, PhD, FACSM; L...

Acute promyelocytic leukemia (APL) is characterized by expression of promyelocytic leukemia (PML)/retinoic acid (RA) receptor A (RARA) protein and all-trans-RA-mediated clinical remissions. RA treatment can confer PML/RARA degradation, overcoming dominant-negative effects of this oncogenic protein. The present study uncovered independent retinoid degradation mechanisms, targeting different doma...