The increasing demand for the development of efficient biocatalysts is a consequence of their broad industrial applications. Typical difficulties that are encountered during their exploitation in a variety of processes are interconnected with factors such as temperature, pH, product inhibitors etc. To eliminate these, research has been directed towards the identification of new enzymes that wou...

Activity-based probes (ABPs) have been used to dissect the biochemical/structural properties and cellular functions of deubiquitinases. However, their utility in studying cysteine-based E3 ubiquitin ligases has been limited. In this study, we evaluate the use of ubiquitin-ABPs (Ub-VME and Ub-PA) and a novel set of E2-Ub-ABPs on a panel of HECT E3 ubiquitin ligases. Our in vitro data show that u...

In this research, the effects of electromagnetic fields of various strengths (0, 1 and 2mT) have been investigated on antioxidant enzymes activity in Valerian (Valeriana officinalis L.). The dry and wet (soaked for 30 min) Valerian seeds were exposed to electromagnetic fields 30 min per day for 3 days. Each treatment and control groups had 3 replicates and 10 seeds were employed for each replic...

The effect of salinity was assessed on the activity of some major antioxidant enzymes i.e., superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), in the seedlings’ roots or shoots of three winter canola (Brassica napus L.) cultivars, Colvert (tolerant), Symbol (semi tolerant) and Agat (susceptible). Seedlings were treated with 0 (control), 50, 100 and 150 mM NaCl for 24 h in hydropoic c...

Eco-enzymes are one of the potential sources for obtaining amylolytic bacterial isolates. The study aims to screen bacteria from eco-enzymes, characterize semi-purification amylase, and identify molecularly using 16S rRNA gene. Screening was carried out by measuring index on a Nutrient agar medium containing 1% tapioca starch. isolate which had highest non-pathogenic selected amylase characteri...

background: enzymes are well known as sensitive catalysts in the laboratory and industrial scale. to improve their properties and for using their significant potential in various reactions as a useful catalyst the stability of enzymes can often require improvement. enzymes immobilization on solid supports such as epoxy- functionalized ferric silica nanocomposite can be effective way to improve ...

Enzymes serve as biocatalysts for innumerable important reactions, however, their application has limitations, which can in many cases be overcome by using appropriate immobilization strategies. Here, a new support for immobilizing enzymes is proposed. This hybrid organic-inorganic support is composed of chitosan-a natural, nontoxic, biodegradable, and edible biopolymer-and sodium polyphosphate...

the aim of the present study was to determine the relationship between histopathological lesions and tissue enzymes in chickens affected by marek's disease.five apparently healthy chickens(as control group)and 25 chickens affected by marek's disease were selected. after consideration of history and gross lesions, tissue samples were collected from kidney, liver, heart, ovary, pectoral...