Lipase-catalyzed production of palm esters by alcoholysis of palm oil with oleyl alcohol in n-hexane was performed in 2L stirred-tank reactor (STR). Investigation on the performance of reactor operation was carried out in batch mode STR with single impeller mounted on the centrally located shaft. Rushton turbine (RT) impellers provide the highest reaction yield (95.8%) at lower agitation speed ...

The kinetics of alcoholysis of methyl propionate and n-propanol catalyzed by Candida antarctica lipase B supported onto silanized Chromosorb P was studied in a continuous solid/gas reactor. In this system the solid phase is composed of a packed enzymatic sample and is percolated by nitrogen as carrier gas, which simultaneously carries substrates to the enzyme while removing reaction products. I...

This study aimed at the investigation of the mechanisms of acidogenesis, which is a key process during anaerobic digestion. To expose possible bottlenecks, specific activities of the key enzymes of acidification, such as acetate kinase (Ack, 0.23-0.99 U mg(-1) protein), butyrate kinase (Buk, < 0.03 U mg(-1) protein) and butyryl-CoA:acetate-CoA transferase (But, 3.24-7.64 U mg(-1) protein), were...

The tripeptide Z-GlyPheLeuNH2 was continuously synthesized in a high yield from three amino acid derivatives, Z-Gly, PheOMe, and LeuNH2, by immobilized thermolysin (IMT) and immobilized alpha-chymotrypsin (IMC) in an organic solvent, ethyl acetate. The optimal conditions for the synthesis of Z-GlyPheOMe were established theoretically. The yield of Z-GlyPheOMe with IMT in ethyl acetate saturated...

Due to the high selectivity and specificity of the enzymatic processes, consuming less energy, and producing less environmental pollution and byproducts, the industrial enzymatic biosynthesis reactors compete with those used for complex chemical synthesis which offen involve a large number of intermediate reaction steps and byproducts. However, chemical engineers have to consider that enzymes h...

KEY WORDS Lipase B from Candida antarctica – Kinetics – transesterification – competitive inhibition – Ping Pong Bi Bi mechanism ABSTRACT The kinetics of alcoholysis of methylpropionate and n-propanol catalyzed by Candida antarctica lipase B supported onto silanized Chromosorb P was studied in a continuous solid/gas reactor. In this system the solid phase is composed of a packed enzymatic sampl...

Amino acid resolutionwas studied in this paper. L-Methionine and DL-methioninewere used as substr for the two snake venom L-amino acid oxidases (from Crotalus adamanteus and Crotalus atrox). E though these enzymes have similar background, they exhibit differences as well as similarities. It found that both enzymes are active towards L-methionine as substrate (Vm = 0.78 mmol min 1 g ) have simil...

Polyvinyl alcohol (PVA) and pectin blends were used to entrap α-amylase (Termamyl) using glutaraldehyde as a cross-linker. The effect of glutaraldehyde concentration (0.25, 0.5, 0.75, 1.0, and 1.25%) on the activity of the immobilized enzyme and rate of enzyme released was tested during a 24 h period. Characteristics of the material, such as scanning electron microscopy (SEM), tensile strength ...

The influence of water on the kinetics of alcoholysis of methyl propionate and n-propanol catalyzed by immobilized lipase B from Candida antarctica was studied in a continuous solid/gas reactor. In this reactor, the solid phase is composed of a packed enzymatic sample which is percolated by gaseous nitrogen, simultaneously carrying gaseous substrates to the enzyme while removing reaction produc...

Enzymes are targets commonly explored in screening assays aiming to discover new leads the drug development process. Among diverse assay models identify enzymatic inhibitors, on-flow based on liquid chromatography (LC) can be highlighted. In these approaches, ligand-enzyme interaction examined by monitoring catalytic activity or affinity/retention. Most applications use biological target immobi...