Herein, we proposed a new electrochemical sensing strategy for T2DM-related SNP detection via DNA-mediated growth of AgNPs on a SWCNT-modified electrode. Coupled with RNase HII enzyme assisted amplification, this approach could realize T2DM-related SNP assay and be applied in crude extracts of carcinoma pancreatic β-cell lines.

Vanadium (V) has become a serious pollutant due to its widespread use in industry. In this study, DNA methylation changes Triticum aestivum L. 'Çetinel 2000' seeds were determined by CRED-RA (Coupled Restriction Enzyme Digestion-Random Amplification) analysis. damage and genomic instability Simple Repeat Inter-Sequence Polymorphism (ISSR) solutions (4.4 mM, 6.6 8.8 mM) boric acid (BA) (4 mM 8 u...

Factor D, also referred to as C3 proactivator convertase, constitutes the activating enzyme of the C3 convertase of the alternative pathway (2) . As such, it is an essential component of initiation and amplification of the pathway (3, 4) . The enzyme cleaves factor B, or C3 proactivator, into the activation fragment Ba (30,000 daltons) and the active site bearing fragment Bb (62,000 daltons) (5...

Enzymatic recognition of SeLNA nucleotides was investigated. KOD XL DNA polymerase was found to be an efficient enzyme in primer extension reactions. Polymerase chain reaction (PCR) amplification of SeLNA-modified DNA templates was also efficiently achieved by Phusion and KOD XL DNA polymerases.

A number of genetic fingerprinting methods have evolved to analyze the population structure and to perform epidemiological and etiological studies of infectious fungi. These methods include multilocus enzyme electrophoresis, restriction fragment-length polymorphism using complex probes, random amplification of polymorphic DNA, and multilocus sequence typing, which are described in this chapter.

A hydrogel-based dual film coating is electrofabricated for transducing bio-relevant chemical information into electronical output. The outer film has a synthetic biology construct that recognizes an external molecular signal and transduces this input into the expression of an enzyme that converts redox-inactive substrate into a redox-active intermediate, which is detected through an amplificat...

A signal amplification based on a combination of hybridization chain reaction (HCR) and enzyme-enhancement was applied in the study of a novel biosensor. The target sequence was added as catalyst which was simply transmitted but did not disappear as the DNA double strand grew on the surface of magnetic beads (MBs).

Nucleic acid amplification testing (NAAT) is increasingly being adopted for diagnosis of Clostridium difficile infection (CDI). Data from 3 states conducting population-based CDI surveillance showed increases ranging from 43% to 67% in CDI incidence attributable to changing from toxin enzyme immunoassays to NAAT. CDI surveillance requires adjustment for testing methods.

Viscosity-sensitive fluorophores, fluorescent molecular rotors based on aminobenzylidene-cyanoacetamide moiety, were tethered to 2'-deoxycytidine triphosphate via a propargylamine linker and incorporated into DNA by polymerases in primer extension, nicking enzyme amplification or PCR. DNA probes incorporating modified nucleosides show a light-up response upon binding to a protein.

We developed a nanofluidics-based enzyme-linked immunosorbent assay (ELISA) device and realized quantification of countable number of molecules by combining enzymatic amplification reaction and ultrasensitive detection with a differential interference contrast thermal lens microscope (DIC-TLM). Our method will contribute to single cell analysis field, in particular for ultrasensitive protein co...