Pectin rich fruit juices need enzymatic treatment for clarification. As fruits are rich in pectin, pectinolytic treatment can efficiently reduce viscosity resulting in clarified fruit juice. Enzymatic treatment of mango pulp results in 80% recovery of total juice present in the fruit. Use of pectin degrading polygalacturonases increases both fruit juice extraction and clarification. Polygalactu...

In the present work, optimization of pectinase-assisted and tri-solvent-mediated extraction of lycopene from waste tomato peels was carried out. The optimized parameters for enzymatic pre-treatment were 2% pectinase concentration, pH 5.5, 4-h incubation, 45 °C and 150 rpm. Maximum recovery of lycopene from tomato peels using optimized tri-solvent extraction was achieved at 45 °C, 120-min incuba...

Abstract Background Mechanochemical-assisted extraction (MCAE) and mechanochemical-assisted enzyme catalysis (MCAEC) methods were developed for enzymatic hydrolysis of calcium phytate from defatted rice bran, respectively. Results The MCAE conditions optimized by response surface methodology: 10% sodium carbonate as the solid reagent, 33 min grinding time, 18:1 ball–material ratio, 330 rpm ball...

Population growth combined with increasingly limited resources of arable land and fresh water has resulted in a need for alternative protein sources. Macroalgae (seaweed) and microalgae are examples of under-exploited "crops". Algae do not compete with traditional food crops for space and resources. This review details the characteristics of commonly consumed algae, as well as their potential f...

In this study, various alkali-pretreated lignocellulose enzymatic hydrolyses were evaluated by using three standard pairs of Miscanthus accessions that showed three distinct monolignol (G, S, H) compositions. Mfl26 samples with elevated G-levels exhibited significantly increased hexose yields of up to 1.61-fold compared to paired samples derived from enzymatic hydrolysis, whereas Msa29 samples ...

Placental estriol in urine can be determined, after enzymatic hydrolysis, by liquid chromatography. A single-step extraction technique results in rapid processing with minimal sample manipulation. Analytical recovery of estriol added to urine was 96 to 106%. Day-to-day precision (CV), established by 10 replicate analyses at a mean concentration of 15.9 mg of estriol per liter, was 3.7%.

BEFORE the introduction of enzymatic zonulysis by Joaquin Barraquer (1958), the normal procedure of senile lens extraction was either by intraor extra-capsular extraction, or, more recently, by the use of the erisophake. This latter operation can be done with much greater facility by the use of the enzyme, as has been demonstrated by Barraquer (1958), Cogan (1958), and Cogan, Symons, and Gibbs ...

We report combination of explants and enzymatic protocol as mixed enzymatic-explant procedure to faster extraction of MSCs from WJ. Umbilical cords (UC) were collected from Imam Khomini Hospital. For explant outgrowth, 6 9 pieces of WJ were transferred onto tissue culture flask and waited for attachment. For mixed enzymatic-explant, 1 cm pieces WJ were placed in enzymatic cocktail comprising 4 ...