A de-acetylase (aryl acylamidase, E.C.3.5.1.13) has been isolated and partially characterized from rat brain. Previous studies have shown that this enzymatic activity is inhibited by low concentrations of serotonin. This report examines the effects of closely related tryptamine derivatives and demonstrates that enzymatic activity is stereospecifically inhibited by LSD. Similar enzymatic activit...

Experiments which combined histochemistry and electron microscopy were performed in studying the sites of enzymatic hydrolysis of thiolacetic acid in the presence of lead ions in diaphragmatic and cardiac muscle. I t was found that in these striated muscles the electron opaque, final product of the histochemical reaction (PbS) was discretely deposited on the swelling of the thick elemental fila...

T4 lysozyme was thought to destroy bacteria by its muramidase activity. However, we demonstrate here that amphipathic helix stretches in the C-terminus of T4 lysozyme mediate its bactericidal and fungistatic activities. In heat-denatured T4 lysozyme, the enzymatic activity is completely abolished but unexpectedly, the antimicrobial functions remain preserved. Small synthetic peptides correspond...

A rapid and accurate method is described for the assay of cholesterol ester hydrolase (CEH) activity. Aliquots of the enzyme-substrate incubation mixture are extracted into isopropanol. The free cholesterol concentration in each extract is determined enzymatically using a single aqueous reagent containing cholesterol oxidase and peroxidase. The free cholesterol remaining after the cholesterol e...

BACKGROUND Non-histone protein acylation is increasingly recognized as an important posttranslational modification, but little is known as to the biochemical properties of protein serine acylating enzymes. METHODOLOGY/PRINCIPAL FINDINGS We here report that we have identified a metal-stimulated serine octanoyltransferase activity in microsomes from human erythroleukemic (HEL) cells. The HEL ac...

Enzymatic activity e.g., pectinase, cellulase, protease and lipase of different fungal pathogens causing stalk rot disease in corn was determined. In-vitro studies; corn stalk rot pathogens viz., Cephalosporium acremonium, Fusarium moniliforme, F. graminearum, F. semitectum, Macrophomina phaseolina, st. 1, M. phaseolina st. 2, Rhizoctonia solani st.1, R. solani st. 2 and Verticillium albo-atrum...