We report a simplified chemically-enhanced enucleation of in vitro matured buffalo oocytes for generating cytoplasts for producing nuclear transfer (NT) embryo production. Oocytes aspirated from the ovaries from abattoir were subjected to in vitro maturation for 22h. In the first experiment, the in vitro matured (IVM) oocytes (22h) were denuded and treated with demecolcine (0.50 μg/ml IVM mediu...

INTRODUCTION Intra-arterial chemotherapy (chemosurgery) for the treatment of retinoblastoma has been performed more than 1600 times (more than 1400 times in Japan and 200 times in New York) over the past 20 years.Despite this treatment's success some eyes cannot be saved and require enucleation. Here we report the histopathologic findings of the remaining intraocular tumor of eyes that were enu...

RATIONALE Enucleation implants are covered with a material that allows the fixation of the extraocular rectus muscles. Usually, the implants are covered in donor sclera, which implies the risk of infection transmission, inflammation and implant rejection, being also an expensive procedure. The new materials used for implant meshing should be tested and a safer and cost effective solution should...

OBJECTIVE To evaluate discordant clinical and pathological diagnoses leading to pediatric enucleations over time. METHODS All pathology reports of pediatric enucleation specimens (subjects aged 0 to 18 years) from 1960 to 2008 were reviewed. Specimens with discordant clinical and pathologic diagnoses were further analyzed. Formalin-fixed, paraffin-embedded sections of enucleated eyes of any m...

The odontogenic keratocyst (OKC) was designated by the World Health Organization (WHO) as a keratocystic odontogenic tumor (KCOT) in 2005. KCOT has been defined as a benign unior multicystic, intraosseous tumor of odontogenic origin, with a characteristic lining of parakeratinized stratified squamous epithelium and potential for aggressive, infiltrative behavior. Additionally, these tumors have...

Orbital exenteration is a disfiguring procedure performed for unresponsive orbital infections and control of recurrent benign tumours and malignancies arising from the eyelids (basal cell carcinoma, squamous cell carcinoma, conjunctival malignant melanoma), lachrymal glands (adenoid cystic carcinoma) or surrounding sinuses. In extremely rare cases the use of a prosthetic eye after enucleation c...

OBJECTIVE To report the clinical and histopathologic findings of retinal astrocytic tumors that showed progressive growth in four patients with tuberous sclerosis complex (TSC). METHODS Four young children each developed an enlarging retinal neoplasm that eventually necessitated enucleation of the affected eye. The systemic findings, clinical course, and histopathologic findings were reviewed...

PURPOSE Changes in anophthalmic socket anatomy can significantly compromise esthetics and motility after enucleation. This study evaluated such changes by using magnetic resonance imaging (MRI) of enucleated and fellow orbits. METHODS High-resolution, surface coil MRI was performed in five patients after enucleation for uveal melanoma. Images were analyzed quantitatively to determine extraocu...

Biliary cystadenoma is very rare non parasitic benign neoplasm of liver with less than 200 cases reported all over the world in the literature. It mainly occurs in middle aged women, slowly progressive; but cannot be differentiated from cystadenocarcinomas before surgery and should always considered Enucleation. Etiology of these tumors is unknown, but several theories have been proposed. Histo...

Indications in the field of pancreatic surgery should be limited considering the technical difficulties and the characteristics of pancreatic diseases. Benign or low-grade malignant tumors, including pseudocysts, islet tumors, and cystic tumors, are indications for distal pancreatectomy. Islet tumors such as insulinomas are good candidates for this procedure when they are located near splenic v...