We previously reported the metabolic 15N labeling of a rat where enrichment ranged from 94% to 74%. We report here an improved labeling strategy which generates 94% 15N enrichment throughout all tissues of the rat. A high 15N enrichment of the internal standard is necessary for accurate quantitation, and thus, this approach will allow quantitative mass spectrometry analysis of animal models of ...

This paper describes the results of a set of measurements carried out to assess the state of the practice of relative uranium enrichment measurements by gamma ray spectrometry and to compare a range of approaches by carrying out measurements on a set of uranium samples with known enrichments. Measurements made by a team from Canberra Industries were carried out using two detector/counting chain...

The EGAN software is a functional implementation of a simple yet powerful paradigm for exploration of large empirical data sets downstream from computational analysis. By focusing on systems-level analysis via enrichment statistics, EGAN enables a human domain expert to transform high-throughput analysis results into hypergraph visualizations: concept maps that leverage the expert’s semantic un...

objective(s):  in our study, graphene oxide-tio2 nanocomposite (go/tio2) was prepared and used for the enrichment of rutin from real samples for the first time.  materials and methods:   the synthesized go/tio2 was characterized by x-ray diffraction, scanning electron microscopy, and ft-ir spectra.  the enrichment process is fast and highly efficient. the factors including contact time, ph, and...

Prostate cancer is one of the most common male malignant neoplasms; however, its causes are not completely understood. A few recent studies have used gene expression profiling of prostate cancer to identify differentially expressed genes and possible relevant pathways. However, few studies have examined the genetic mechanics of prostate cancer at the pathway level to search for such pathwa...

The microflora developing during a continuous enrichment culture from a hydrothermal chimney sample was investigated by molecular methods. The culture was performed in a gas-lift bioreactor under anaerobic conditions, at 90°C and pH 6.5, on a complex medium containing sulfur as the terminal electron acceptor. Archaeal and bacterial diversity was studied. Microorganisms affiliated with the gener...

Although currently available strategies for the preparation of exome-enriched libraries are well established, a final validation of the libraries in terms of exome enrichment efficiency prior to the sequencing step is of considerable importance. Here, we present a strategy for the evaluation of exome enrichment, i.e., the Multipoint Test for Targeted-enrichment Efficiency (MTTE), PCR-based appr...

the aim of this study was to determine the extent of metal pollutions and the identification of their major sources in the vicinity of the sangan iron mine occurring in ne iran. soil samples were collected from the vicinity of the mine site and analyzed for heavy metals. in addition, the chemical speciation of these metals was investigated by means of the sequential extraction procedure. the st...

In this study, we developed a platform that can be used to rapidly enrich polyhistidine(His)-tagged proteins/peptides from complex samples selectively using the Fe3O4@Al2O3 magnetic nanoparticles (MNPs) as the affinity probes. At pH 7, the dissociation constant between poly-His, i.e., His6, and the Fe3O4@Al2O3 MNPs was ~10(-5) M and the trapping capacity was ~100 nmol/mg for His6. Enrichment wa...

A routine approach to inferring functions for a gene set is by using function enrichment analysis based on GO, KEGG or other curated terms and pathways. However, such analysis requires the existence of overlapping genes between the query gene set and those annotated by GO/KEGG. Furthermore, GO/KEGG databases only maintain a very restricted vocabulary. Here, we have developed a tool called "CoCi...