نتایج جستجو برای: electroporation
تعداد نتایج: 4856 فیلتر نتایج به سال:
Quantum dots (QDs) have found a wide range of biological applications as fluorophores due to their extraordinary brightness and high photostability that are far superior to those of conventional organic dyes. These traits are particularly appealing for studying cell biology under a cellular autofluorescence background and with a long observation period. However, it remains the most important op...
Delivery of nucleic acids into animal tissues by electroporation is an appealing approach for various types of gene therapy, but efficiency of existing methodsis not satisfactory. Here we present the validation of novel electroporation patch (ep-Patch) for efficient delivery of DNA and siRNA into mouse tissues. Using micromachining technology, closely spaced gold electrodes were made on the pli...
Electroporation is the formation of permeabilizing structures in the cell membrane under the influence of an externally imposed electric field. The resulting increased permeability of the membrane enables a wide range of biological applications, including the delivery of normally excluded substances into cells. While electroporation is used extensively in biology, biotechnology, and medicine, i...
Electroporation has been widely used in delivering foreign biomolecules into cells, but there is still much room for improvement, such as cell viability and integrity. In this manuscript, we investigate the distribution and the toxicity of pH changes during electroporation, which significantly decreases cell viability. A localized pH gradient forms between anode and cathode leading to a localiz...
Screening for a molecular target for cancer therapy requires multiple steps, of which an important one is evaluation of the knockdown effect of the target molecule on pregrown xenograft tumors. However, methods currently used for local administration of knockdown reagents, such as short interfering RNA (siRNA), are not satisfactory as to simplicity and efficiency. We established an electroporat...
propose: standard electroporation and electrochemotherapy caused the endothe- lial cell permeability and reduction in tumor blood flow. the effects of low voltage and high frequency electroporation on the endothelial cells permeability and viability were expected. therefore, the propose of present study was to evaluate the effect of electroporation with bleomycin or alone on viability and perme...
introduction: vaccinology provides promising approaches for the control of various infectious diseases. among different strategies, dna vaccines offer attractive research opportunities for development of vaccines for induction of antigen-specific immunity owing to their stability, simplicity of delivery, safety and cost effectiveness. however, there is a need to increase their potency by the us...
Biological membranes form transient, conductive pores in response to elevated transmembrane voltage, a phenomenon termed electroporation. These pores facilitate electrical and molecular transport across cell membranes that are normally impermeable. By applying pulsed electric fields to cells, electroporation can be used to deliver nucleic acids, drugs, and other molecules into cells, making it ...
* Correspondence: Damijan. [email protected] University of Ljubljana, Faculty of Electrical Engineering, Ljubljana, 1000, Slovenia When a cell is exposed to a sufficiently intense electric field (for a sufficient duration), local defects in the cell membrane appear and become permeable to molecules that otherwise cannot pass through it. This phenomenon is commonly known as electroporation ...
Sterilization using high-intensity electric fields is detrimental to health if safety inadequate, so it necessary study the possibility of sterilization low-intensity fields. This aims determine lowest field intensity and treatment time deactivate bacteria that make up biofilms explain mechanism inactivation. The samples were from Pseudomonas aeruginosa Staphylococcus epidermidis grown on cathe...
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