The aims were to evaluate the suitability of a non-commercial TRIS-lecithin (LC) extender and the effect of different concentrations of catalase (CAT) on motility, capacitation status (Chlortetracycline-assay) and zona pellucida (ZP) binding capacity of canine spermatozoa stored at +5 degrees C for 4 days. The sperm-rich fractions of the ejaculates of four stud dogs were divided into four aliqu...

To define a model for enzyme latency in vitro, the capture was studied of egg white lysozyme by liposomes (phospholipid spherules in the smectic mesophase). Gel filtration resolved liposomes formed in the absence of enzyme from free enzyme when both were chromatographed immediately after mixing. In contrast, if liposomes were swollen in the presence of lysozyme, subsequent chromatography disclo...

as the antigen for a simplified precipitation test for syphilis. Cardiolipin is the lipid which, in combination with lecithin, gives the alcoholic extract of beef heart its antigenic properties. It is a complex phosphatidic acid with a molecular weight of 2195 and iodine number 120-125. It requires lecithin and cholesterol for antigenicity. It has not yet been synthesized. The cardiolipin-lecit...

Human platelets incubated with 32p lysolecithin convert this compound to platelet lecithin and the water-soluble product glycerylphosphorylcholine. Lecithin synthesis from lysolecithin by intact platelets has a pH optimum of 9, whereas platelet homogenates show a pH optimum of 7.5 for the same reaction. Conversion of lysolecithin to platelet lecithin takes place by direct acylation of lysolecit...

BACKGROUND Cationic nanoemulsions have been recently considered as potential delivery systems for nucleic acids. This study reports the influence of phospholipids on the properties of cationic nanoemulsions/DNA plasmid complexes. METHODS Nanoemulsions composed of medium-chain triglycerides, stearylamine, egg lecithin or isolated phospholipids, ie, DSPC, DOPC, DSPE, or DOPE, glycerol, and wate...

Lecithinase can be determined by the measurement of the opalescence developing in serum (Nagler, 1939; Seifert, 1939), the precipitation of lecithovitellin (van Heyningen, 1941a; Weed et al., 1942; Theriault, 1945), the release of hemoglobin from erythrocytes (van Heyningen, 1941b; Burrows, 1951), the determination of the amount of acid soluble phosphorus released from lecithin (MacFarlane and ...

ketorolac tromethamine (kt) containing microemulsion-based gels (mbgs) have been formulated, based on the previous phase diagram studies, using a pharmaceutically acceptable surfactant (soybean lecithin; epikuron 200) and oil (isopropyl myristate; ipm) and the effect of formulation variables on the release profile of the drug from mbgs through intact guinea pig skin and various artificial membr...

in a 42-d experiment, 576 one-day-old vencobb 308 broiler chicks were used to investigate the effects of lecithin extract (0.5 g/kg), choline chloride 60% (1 g/kg) and bio choline (1 g/kg) in diets of moderate and high energy in a 4 × 2 factorial arrangement on performance and certain physiological traits in broiler chickens. the inclusion of bio choline and lecithin extract in the diet signifi...

Background: Egg yolk is the main cryoprotectant agent in semen freezing extenders which is used in order to protect spermatozoa against cold shock. However, elimination of animal bioproducts from the cryopreservation protocol is becoming mandatory. Therefore, the aim of this study is to compare a previously studied, homemade soya bean lecithin based extender with a commercially available extend...