dynamic light scattering

The measurement of light scattering is a well established scientific method for the analysis of particle sizes in macromolecular solutions [1]. The spectrum of applications reaches from physics, over pharmacology, molecular and cell biology, immunology right up to environmental research. The underlying principle of this approach is the extraction of spectral information from light scattering us...

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Analysis of FtsZ assembly by light scattering and determination of the role of divalent metal cations.

Journal: :Journal of bacteriology 1999

A Mukherjee J Lutkenhaus

FtsZ is an ancestral homologue of tubulin that polymerizes in a GTP-dependent manner. In this study, we used 90 degrees angle light scattering to investigate FtsZ polymerization. The critical concentration for polymerization obtained by this method is similar to that obtained by centrifugation, confirming that the light scattering is proportional to polymer mass. Furthermore, the dynamics of Ft...

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