The recA protein of Escherichia coli promotes the complete exchange of strands between full-length linear duplex and single-stranded circular phi X174 DNA molecules. Analysis of the reaction by electron microscopy confirms that D loops containing short heteroduplex regions are rapidly formed at the ends of the linear duplex, followed by a relatively slow branch migration that converts the D loo...

A conformational distortion in the DNA duplex at the regulatory region of human papillomavirus type-11 next to an intermolecular triplex, formed with a synthetic oligonucleotide, was investigated with several chemical probes. The sequence targeted for triplex formation borders on the binding sites for the regulatory proteins encoded by the viral E2 open reading frame. Dimethyl sulfate, diethyl ...

The synthesis and properties of fully modified 4'-thioDNAs, oligonucleotides consisting of 2'-deoxy-4'-thionucleosides, were examined. In addition to the known literature properties (preferable hybridization with RNA and resistance to endonuclease hydrolysis), we also observed higher resistance of 4'-thioDNA to 3'-exonuclease cleavage. Furthermore, we found that fully modified 4'-thioDNAs behav...

Thermodynamic parameters are reported for duplex formation in 1 M NaCl for 16 RNA sequences, each containing a core tetramer duplex, GGCC, and a 3' overhang consisting of two bases. The results indicate additional double-helical stability is conferred by the double 3' terminal overhang relative to the single 3' terminal overhang. A nearest-neighbor analysis of the data indicates that the free e...

BACKGROUND Duplex kidney is a common anomaly that is frequently associated with multiple complications. Typical computed tomography urography (CTU) includes four phases (unenhanced, arterial, parenchymal and excretory) and has been suggested to considerably aid in the duplex kidney diagnosi. Unfortunately, regarding duplex kidney with prolonged dilatation, the affected parenchyma and tortuous u...

The recA protein from Escherichia coli can homologously align two duplex DNA molecules; however, this interaction is much less efficient than the alignment of a single strand and a duplex. Three strand paranemic joints are readily detected. In contrast, duplex-duplex pairing is detected only when the incoming (second) duplex is negatively supercoiled, and even here the pairing is inefficient. T...

Joint communication and sensing (JCAS) is a very promising 6G technology, which attracts more research attention. Compared with communication, radar has many unique features in terms of waveform design criteria, selfinterference cancellation (SIC), aperture-dependent resolution, virtual aperture. This paper proposes novel named max-aperture slicing (MaRS) to gain large time-frequency aperture, ...

Embedded systems play an important role in our daily lives: We interact, sometimes unknowingly, with embedded systems in applications ranging from cars, trains, and aircraft to washing machines and other household appliances. In an increasing number of applications, the system’s ability to detect or tolerate transient and permanent faults is important. To achieve this goal, a system requires re...

Hardware duplication techniques are widely used for concurrent error detection in dependable systems to ensure high availability and data integrity. These techniques are vulnerable to common-mode failures (CMFs). Use of duplex systems with diverse implementations of the two modules has been proposed in the past for protection against CMFs. In this paper, we define a category of faults, called n...