This paper describes the development of an HPLC-UV-MS method for quantitative determination of andrographolide and dehydroandrographolide in Andrographis Herba and establishment of its chromatographic fingerprint. The method was validated for linearity, limit of detection and quantification, inter- and intra-day precisions, repeatability, stability and recovery. All the validation results of qu...

Parmeliaceae represents the largest and widespread family of lichens and includes species that attract much interest regarding pharmacological activities, due to their production of unique secondary metabolites. The current work aimed to investigate the in vitro antioxidant and cytotoxic activities of the methanol extracts of ten Parmeliaceae species, collected in different continents. Methanol...

OBJECTIVES The purpose of this study was to compare the antioxidant and anti-inflammatory effects of Alum (AL) and Burnt Alum (BAL), which are commonly used as external ointments. METHODS Extracts of AL and BAL were classified into three groups: 20, 50, and 100 ㎎/㎕. The cytotoxicity was measured by using MTT assays in human keratinocyte cell line (HaCaT). The anti-oxidant effect was measured ...

This study aimed to determine total antioxidant and antioxidant activity of selected local varieties of lettuce (Lactuca sativa L.). Five varieties (iceberg, butterhead, romaine, green coral and red coral) were subjected to DPPH radical scavenging activity and ferric reducing antioxidant power assay (FRAP) assays for determination of antioxidant activity. Total phenolic content and total flavon...

SCOPE Dietary flavonoids and stilbenes are important polyphenols in foods, such as, e.g. fruits, vegetables, nuts, and tea as they are of great interest for their bioactivities, which are related to the anti-oxidative property. METHODS AND RESULTS The relationship between the structural properties of dietary polyphenols and their affinities for milk proteins (MP) was investigated. Methylation...

The stone fish (Actinopyga lecanora) ethanolic and methanolic tissue extracts were investigated for total phenolic contents (TPCs) as well as antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH(•)) radical scavenging activity and ferric reducing antioxidant power (FRAP) assays. Both extracts showed low amount of phenolics (20.33 to 17.03 mg of gallic acid equivalents/100 g dried samp...

Plant foods are important due to their antioxidant activity (AOA) attributed to the phenolics which are known to protect organisms against harmful effects of oxygen radicals. However, information on antioxidant activity of Indian plant foods is scanty. Therefore, the present study evaluated the AOA of cereals, millets, pulses and legumes, commonly consumed in India and assessed the relationship...

Objective: The present investigation was an attempt to evaluate the phytochemical constituents, free radical scavenging activities and antioxidant properties of the methanol extract of leaves of Hypericum hookerianum. Methods: The total antioxidant activity was assayed by DPPH free radical scavenging assay method. The phytochemicals in the methanol extract of leaves of Hypericum hookerianum wer...

The DPPH radical scavenging activity of 2',4',6'-trihydroxy- and 2'-hydroxy-4',6'-dimethoxychalcones carrying a 2,3- and 3,4-dihydroxylated, and 3,4,5-trihydroxylated B-ring was evaluated in alcoholic and non-alcoholic solvents. All test compounds scavenged more than two equivalent of radicals by a possible conversion to the corresponding B-ring quinones and in most cases subsequently underwent...

Despite strawberry tree (Arbutus unedo L.) leaves had a long use in traditional medicine due to its antiseptic, diuretic, astringent and depurative properties, the potential of their antioxidant activity are still lacking. Our study goals to assess the antioxidant and free radical scavenging potential of water, ethanol, methanol and diethyl ether extracts of A. unedo leaves. Total phenols conte...