PURPOSE Leptospirosis is a zoonotic disease with humans getting the infection either from rodent hosts or from domestic animals. Urine contaminated environment is the common source of infection. This is an under-reported disease in Andhra Pradesh. We report a retrospective hospital-based study on 55 patients with suspected leptospirosis. METHODS A total of 55 serum samples were collected from...

ELISA, cDNA dot blot hybridization and transmission by vector aphids were used to investigate the occurrence and degree of cross-protection produced in oat plants by virus isolates representing five strains or serotypes of barley yellow dwarf virus, namely PAV, MAV, SGV, RPV and RMV. Generally, the degree of cross-protection was positively correlated with the serological relatedness between the...

BACKGROUND The identity of allergenic almond proteins is incomplete. OBJECTIVE Our objective was to characterize patient IgE reactivity to a recombinant and corresponding native almond allergen. METHODS An almond cDNA library was screened with sera from patients with allergy for IgE binding proteins. Two reactive clones were sequenced, and 1 was expressed. The expressed recombinant allergen...

ABSTRACT Dot-ELISA test is developed in mice models to identify cobra and viper snake species in snake bite cases. Simplicity and cost effective qualities of this test showed great promises to be utilized in the field which can be useful for farmers, plantation workers and fisherman who are working in field and become victim of snake bite cases. Dot-ELISA test can be an alternative solution to ...

BACKGROUND The inefficiency of the current tachyzoite antigen-based serological assays for the serodiagnosis of Toxoplasma gondii infection mandates the need for acquirement of reliable and standard diagnostic reagents. Recently, epitope-based antigens have emerged as an alternative diagnostic marker for the achievement of highly sensitive and specific capture antigens. In this study, the diagn...

Visceral leishmaniasis, caused by Leishmania (Leishmania) chagasi, is a chronic parasitic disease of humans and dogs. Confirmation of the protozoal agent in bone marrow, lymph node or spleen aspirate is diagnostic, while specific-IgG serology is used mainly for epidemiology despite the general presence of high levels of serum immunoglobulin. Anecdotal reports of false-negative serology in activ...

We demonstrated the presence of circulating Abs from schizophrenic patients able to interact with cerebral frontal cortex-activating muscarinic acetylcholine receptors (mAChR). Sera and purified IgG from 21 paranoid schizophrenic and 25 age-matched normal subjects were studied by indirect immunofluorescence, flow cytometry, immunoblotting, dot blot, ELISA, and radioligand competition assays. Ra...

chlamydia trachomatis is one of the main causes of sexually transmitted diseases (stds) such as  prostatitis and epididymitis in men and cervicitis, endometriosis, vaginitis and ureogenital tract infections in women.  serological tests with sensitivities related to specific antigens are commonly used as  routine laboratory tests for diagnosis of chlamydia. in this research the chlamydia major o...

lactoferrin (lf) has antimicrobial properties against bacteria, fungi and several viruses including herpes virus, hiv and hepatitis c virus. the aim of this study was to detect lf in pmns and plasma of the patients suffering from hepatitis c and the healthy persons. the sonicated solutions of pmns of two groups were evaluated by sds-page (10%), isoelectric focusing (30%) and dot blotting .the l...

Objective Dot Blot (DB) assay provides highly specific results, but usually is not reliable for quantification of antibody production. The need for a more objective DB assay to provide a better definition of the immune status, against HIV antigens, promoted this study to develop a quantitative DB assay. Materials and Methods Dot blot (DB) strips for antibodies, directed to human immunodeficienc...