نتایج جستجو برای: dna dsb

تعداد نتایج: 507900  

2017
Pingping Wang Stephanie Byrum Faith C. Fowler Sangita Pal Alan J. Tackett Jessica K. Tyler

Here, we use ChAP-MS (chromatin affinity purification with mass spectrometry), for the affinity purification of a sequence-specific single-copy endogenous chromosomal locus containing a DNA double-strand break (DSB). We found multiple new histone post-translational modifications enriched on chromatin bearing a DSB from budding yeast. One of these, methylation of histone H3 on lysine 125, has no...

2010
Woo-Hyun Chung Zhu Zhu Alma Papusha Anna Malkova Grzegorz Ira

The formation of single-stranded DNA (ssDNA) at double-strand break (DSB) ends is essential in repair by homologous recombination and is mediated by DNA helicases and nucleases. Here we estimated the length of ssDNA generated during DSB repair and analyzed the consequences of elimination of processive resection pathways mediated by Sgs1 helicase and Exo1 nuclease on DSB repair fidelity. In wild...

Journal: :Journal of cell science 2011
Duen-Wei Hsu Rhian Kiely C Anne-Marie Couto Hong-Yu Wang Jessica J R Hudson Christine Borer Catherine J Pears Nicholas D Lakin

DNA double-strand breaks (DSBs) can be repaired by homologous recombination (HR) or non-homologous end joining (NHEJ). The mechanisms that govern whether a DSB is repaired by NHEJ or HR remain unclear. Here, we characterise DSB repair in the amoeba Dictyostelium. HR is the principal pathway responsible for resistance to DSBs during vegetative cell growth, a stage of the life cycle when cells ar...

Journal: :Blood 2012
Roni Nowarski Ofer I Wilner Ori Cheshin Or D Shahar Edan Kenig Leah Baraz Elena Britan-Rosich Arnon Nagler Reuben S Harris Michal Goldberg Itamar Willner Moshe Kotler

APOBEC3 proteins catalyze deamination of cytidines in single-stranded DNA (ssDNA), providing innate protection against retroviral replication by inducing deleterious dC > dU hypermutation of replication intermediates. APOBEC3G expression is induced in mitogen-activated lymphocytes; however, no physiologic role related to lymphoid cell proliferation has yet to be determined. Moreover, whether AP...

2011
F. Natale A. Rapp W. Chen

Heavy ions are extensively used at GSI as a tool to study DNA repair processes and chromatin structure dynamics. Ionizing radiation (IR) produces DNA double strand breaks (DSB) triggering the DNA damage response (DDR). One of the earliest events of DDR consists of the extensive phosphorylation of H2AX histone variant (γH2AX), whose distribution throughout the genome appears to be non-random. γH...

2015
Xuan Zhu Scott Keeney

Running title: Transcription factors and meiotic double-strand breaks ABSTRACT Meiotic recombination initiates with DNA double-strand breaks (DSBs) made by Spo11. In Saccharomyces cerevisiae, many DSBs occur in " hotspots " coinciding with nucleosome-depleted gene promoters. Transcription factors (TFs) stimulate DSB formation in some hotspots, but TF roles are complex and variable between locat...

2012
Volker Dahmen Ralf Kriehuber

PURPOSE Triplex-forming oligonucleotides (TFO) bind to the DNA double helix in a sequence-specific manner. Therefore, TFO seem to be a suitable carrier for Auger electron emitters to damage exclusively targeted DNA sequences, e.g., in tumor cells. We studied the influence of I-125 labeled TFO with regard to cell survival and induction of DNA double-strand breaks (DSB) using TFO with different g...

2016
Sarah-Felicitas Himmels Alessandro A. Sartori

DNA double-strand breaks (DSBs) are one of the most detrimental lesions, as their incorrect or incomplete repair can lead to genomic instability, a hallmark of cancer. Cells have evolved two major competing DSB repair mechanisms: Homologous recombination (HR) and non-homologous end joining (NHEJ). HR is initiated by DNA-end resection, an evolutionarily conserved process that generates stretches...

2012
Yvonne Lorat Stefanie Schanz Nadine Schuler Gunther Wennemuth Christian Rübe Claudia E. Rübe

PURPOSE DNA double-strand breaks (DSBs) generated by ionizing radiation pose a serious threat to the preservation of genetic and epigenetic information. The known importance of local chromatin configuration in DSB repair raises the question of whether breaks in different chromatin environments are recognized and repaired by the same repair machinery and with similar efficiency. An essential ste...

Journal: :International journal of radiation biology 1996
M Villalobos D Becerra M I Núñez M T Valenzuela E Siles N Olea V Pedraza J M Ruiz de Almodóvar

Treatments which inhibit or retard progression of the cell through the cell cycle have been reported to reduce the effectiveness of ionizing radiation by increasing cellular radioresistance. We studied cellular radiosensitivity and radiation-induced DNA damage (double-strand break, dsb) in both hormone-sensitive and non-sensitive human breast cancer cell lines. After 72h of culture in an oestra...

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