Twenty one bacterial isolates were isolated from methyl parathion treated agricultural soil from in and around the Gwalior city, Madhya Pradesh to study the degradation of Methyl Parathion. Isolation was done by enrichment in Basal mineral medium with 50 mg/L methyl parathion as sole carbon and energy source. No supplement as a carbon and phosphorus source was added in medium. Methyl parathion ...

In order to overproduce bioinsecticides production by a sporeless Bacillus thuringiensis strain, an optimal composition of a cheap medium was defined using a response surface methodology. In a first step, a Plackett-Burman design used to evaluate the effects of eight medium components on delta-endotoxin production showed that starch, soya bean and sodium chloride exhibited significant effects o...

Earlier investigations showed a positive correlation between basal cytosolic free calcium in human platelets and blood pressure; however, recent studies have failed to show this relation. We undertook the present work to examine which platelet cytosolic calcium parameters (namely, cytosolic calcium in resting or stimulated states in calcium-containing and calcium-free media) present the least v...

level: intermediAte Most biopharmaceutical production platforms are based on fed-batch cell culture protocols, which can support high volumetric productivity while maintaining low operational complexity (1). The industry is interested in developing or refining high-titer cell culture processes to meet increasing market demands and reduce manufacturing costs (2). Although advancements in cell en...

The regulation of renin release is unusual in that intracellular calcium reportedly acts as an inhibitory second messenger. Increased calcium not only inhibits renin release but is a cofactor in nitric oxide synthesis. Also, nitric oxide can inhibit renin release. This study was done in vitro using rat renal cortical slices to determine whether calcium-mediated renin inhibition could be in part...

During an investigation of the effects of nutrients on formic hydrogenlyase activity in Escherichia coli, it was noted that the harvested cells possessed no measurable formic hydrogenlyase activity when grown in a basal medium composed of inorganic salts and glucose and containing NH4NOs as the sole source of nitrogen. This was in agreement with the data reported in a previous communication (Bi...

The effect of plant growth regulators on shoot proliferation from shoot tip explants of Ajuga multiflora was studied. The highest number of shoots (17.1) was observed when shoot tip explants were cultured on Murashige and Skoog (MS) medium fortified with 8.0 µM 6-Benzyladenine (BA) and 2.7 µM α-naphthaleneacetic acid (NAA). The mean number of shoots per explant was increased 1.6-fold in liquid ...

Mouse epidermal basal cells can be cultured in medium with a Ca2+ concentration below 0.1 mM and induced to terminally differentiate in medium with higher Ca2+. Infection of basal cells with Kirsten or Harvey sarcoma virus causes a marked stimulation of basal cell proliferation. Challenge of infected basal cell cultures with 0.5 mM Ca2+ medium indicates that terminal differentiation is altered,...