In order to research the application of [C4mim] Br-K2HPO4 ionic liquid aqueous two-phase system to extract ficin, the influence of reagents to the activity of ficin have been investigated. This study aimed to investigate the effects of species and concentration of the ionic liquid, the dosage of ficin, pH value and temperature on the partitioning behavior of ficin, respectively. Response surfac...

Dispersive liquid-liquid microextraction (DLLME) coupled with gas chromatography-mass spectrometry (GC-MS) has been developed for preconcentration and determination of tramadol, ((±)-cis-2-[(dimethylamino)methyl]-1-(3-methoxyphenyl)cyclohexanol-HCl), in aqueous and biological samples (urine, blood). DLLME is a simple, rapid and efficient method for determination of drugs in aqueous samples. Eff...

Microwave-assisted extraction of pectin from lemon peels by using ionic liquid as alternative solvent was investigated. The extracted pectin was detected by Fourier transform infrared spectra. The extraction conditions were optimized through the different experiments in conjunction with the response surface methodology. A pectin yield of 24.68 % was obtained under the optimal parameters: the ex...

abstract: in this study, an analytical method is developed to determine 15 polycyclic aromatic hydrocarbons (pahs) in olive and refined pomace olive oils using hplc coupled with a fluorescence detector. the standardised method of ultrasound-assisted solvent extraction consisted of liquid–liquid extraction with organic solvent and purification on c18 and florisil bonded-phase cartridges was modi...

Consider how these problems could be solved by using a solid phase (such as a chromatographic sorbent) instead of the second liquid phase. There are still two distinct phases that can be readily segregated. Also, by proper selection of pH and type of solid phase sorbent, one could optimize the conditions to selectively partition the component(s) of interest on the solid phase. After removal of ...

spectrometric techniques for the analysis of trace lead have developed rapidly due to the increasing need for accurate measurements at extremely low levels of this element in diverse matrices. this review covers separation and preconcentration procedures, and considers the features of the application with several spectrometric techniques. the use of an appropriate sample handling technique is a...

In this study, a dispersive liquid–liquid microextraction method using an extraction solvent lighter than water has been developed for the extraction and preconcentration of cholecalciferol and calcifediol from plasma samples followed by high performance liquid chromatography determination. Initially, acetonitrile and sodium chloride (NaCl) are added into the plasma as an extraction solvent and...

Amongst organophosphate pesticides, the one most widely used and common environmental contaminant is diazinon; thus methods for its trace analysis in environmental samples must be developed. Use of diazinon imprinted polymers such as sorbents in solid phase extraction, is a prominent and novel application area of molecular imprinted polymers. For diazinon extraction, high performance liquid chr...

A simple and solvent-minimized sample preparation technique based on hollow fibre-protected liquid-phase micro-extraction has been developed for extraction of fifteen organic sulphur compounds from aqueous samples. The analysis of the extracted them was performed by gas chromatography equipped with mass spectrometry and/or flame photometric detectors. 3.3 µL of organic solvent located in the lu...