نتایج جستجو برای: direct sequencing

تعداد نتایج: 542795  

احمدی, محمدحسین, امیری‌‌زاده, ناصر, ساروخانی, محمدرضا,

   Background and Aim: About 13 beta-globin mutations encompass 70-90% of the mutation spectrum in Iran. These mutations are called common beta-globin mutations. The rest are rare or unknown mutations. The objective of this study was to identify the rare or unknown beta-globin mutations in Qazvin province.   Materials and Methods: In this descriptive-analytic study, EDTA-containing venous blood...

Journal: :jundishapur journal of microbiology 0
bahram nasr esfahani department of microbiology, isfahan university of medical sciences, isfahan, ir iran fatemeh sadat zarkesh department of microbiology, science and research branch, islamic azad university, fars, ir iran hadi rezaei yazdi department of microbiology, jahrom university of medical sciences, jahrom, ir iran tooba radaee department of microbiology, isfahan university of medical sciences, isfahan, ir iran; department of microbiology, isfahan university of medical sciences, isfahan, ir iran. tel: +98-3137922493, fax: +98-3136688597

conclusions we concluded from the results that the frequency of emb-resistant m. tuberculosis cases in iran is lower than that of many other regions. the pcr-sscp technique can separate resistant isolates from sensitive isolates. the sequencing results of this study showed mutation in codons 309 and 299 of the embb gene. in none of the resistant isolates, mutation was observed in codon 306. fur...

Ardeshir Qaderi Darab Yazdani Tan Yee How Zainal Abidin Mior Ahmad

30 milled rice samples were collected from retailers in four states of Malaysia. These samples were evaluated for Eurotium spp. contaminations by direct plating on malt extract salt agar (MESA). All Eurotium were isolated and identified based on morphology and nucleotide sequences of internal transcribed spacer 1 (ITS1) and ITS2 of the rDNA.  Four Eurotium species (E. rubrum, E. amstelodami, E....

In the current study, we conducted a mutation screening of tumor-associated calcium signal transducer 2 (TACSTD2) gene in six consanguineous Iranian families with gelatinous drop-like corneal dystrophy (GDLD), in order to find the causative mutations. Detailed eye examination was performed by ophthalmologist to confirm GDLD in patients. To detect the possible mutations, direct Sanger sequencing...

Journal: :CNS neuroscience & therapeutics 2013
Hong-Fu Li Wang Ni Zhi-Qi Xiong Jianfeng Xu Zhi-Ying Wu

AIMS PRRT2 was recently identified as a causative gene for paroxysmal kinesigenic dyskinesia (PKD), and the c.649dupC mutation was shown to be a "high frequency" mutation. This mutation was also identified in many sporadic cases. This might be attributed to the incomplete penetrance of c.649dupC. Alternatively, c.649dupC might derive from de novo. The aim of this study is to elucidate the possi...

Journal: :Biotechnology 1988
M A Innis K B Myambo D H Gelfand M A Brow

The highly thermostable DNA polymerase from Thermus aquaticus (Taq) is ideal for both manual and automated DNA sequencing because it is fast, highly processive, has little or no 3'-exonuclease activity, and is active over a broad range of temperatures. Sequencing protocols are presented that produce readable extension products greater than 1000 bases having uniform band intensities. A combinati...

2014
Marta Brozynska Agnelo Furtado Robert James Henry Hikmet Budak

Direct sequencing of total plant DNA using next generation sequencing technologies generates a whole chloroplast genome sequence that has the potential to provide a barcode for use in plant and food identification. Advances in DNA sequencing platforms may make this an attractive approach for routine plant identification. The HiSeq (Illumina) and Ion Torrent (Life Technology) sequencing platform...

Journal: :Nucleic acids research 1994
E Rouer

Denaturation of the double stranded DNA is required before sequencing by the dideoxy chain termination method (1). Good results are routinely obtained with the alkaline-denaturation method, which involves incubation of the DNA in the presence of 0.2 M NaOH for a few minutes at room or higher temperatures (2,3). The alkaline mixture is then neutralized by the addition of sodium acetate (0.3 M), ...

Journal: :Nucleic acids research 1999
K R Mitchelson J Drenth H Duong J X Chaparro

Random amplified polymorphic DNA (RAPD) markers are used widely to develop high resolution genetic maps and for genome fingerprinting. Typically, single oligomers of approximately 10 nucleotides are used to PCR amplify characteristic RAPD marker fragments. We describe an efficient method for the direct end-sequencing of gel-purified RAPD fragments using one primer from a set of four 3'-terminal...

Journal: :Journal of clinical microbiology 2012
Nima Mosammaparast Alexander J McAdam Frederick S Nolte

Over the past decade, there has been an explosion in the use of molecular tests to diagnose and manage infectious diseases. HIV is a prime example of an infectious agent whose diagnosis at least in the acute stage, susceptibility testing, and management are all dependent on molecular diagnostics. The ability to accurately diagnose a plethora of respiratory pathogens quickly, simply, and relativ...

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