نتایج جستجو برای: digoxigenin

تعداد نتایج: 744  

Journal: :Investigative ophthalmology & visual science 2000
D M Gamm L K Barthel P A Raymond M D Uhler

PURPOSE To examine the expression of the major isoforms of cyclic guanosine monophosphate (cGMP)-dependent protein kinase (cGK) in mouse eye. METHODS Immunohistochemical localization of cGMP in mouse eye cryosections was performed using an anti-cGMP antibody, followed by visualization with indirect fluorescence microscopy. The presence of types Ialpha, Ibeta, and II cGK mRNAs in mouse eye ext...

Journal: :The Journal of pharmacology and experimental therapeutics 2015
Letteria Minutoli Pietro Antonuccio Natasha Irrera Mariagrazia Rinaldi Alessandra Bitto Herbert Marini Gabriele Pizzino Carmelo Romeo Antonina Pisani Giuseppe Santoro Domenico Puzzolo Carlo Magno Francesco Squadrito Antonio Micali Domenica Altavilla

We investigated the role of the nucleotide-binding oligomerization domain (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome during testis ischemia and reperfusion injury (TI/R) in wild-type (WT) and NLRP3 knock-out (KO) mice. WT and KO mice underwent 1 hour testicular ischemia followed by 4 hours and 1 and 7 days of reperfusion or a sham TI/R. Furthermore, two groups of ...

Journal: :The Journal of neuroscience : the official journal of the Society for Neuroscience 1999
J K Cui C Y Hsu P K Liu

We examined the uptake and distribution of an antisense phosphorothioated oligodeoxynucleotide (s-ODN) to c-fos, rncfosr115, infused into the left cerebral ventricle of male Long-Evans rats and the effect of this s-ODN on subsequent Fos, NGF, neurotrophin-3 (NT-3), and actin expression. To establish the uptake and turnover of s-ODN in the brain, we studied the copurification of the immunoreacti...

Journal: :Journal of clinical pathology 1997
C Walters D G Powe C J Padfield D G Fagan

AIMS To compare the application of a non-radioactive in situ hybridisation (ISH) technique with an immunocytochemical technique for the detection of human parvovirus B19 in formalin fixed, paraffin wax embedded sections of macerated fetal tissue. METHODS Archived samples of liver, lung or kidney from 19 human fetuses were investigated for parvovirus B19 using a full length digoxigenin labelle...

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