Objective: After traumatic brain injury (TBI), postural instability often results. This can effect ambulation and, subsequently, activities of daily living. Various forms of rehabilitation have been developed, but few offer engaging activities that provide informative dynamic balance training. The goal of this pilot study was to build a goal-based system that provided visual biofeedback to crea...

Cultivars of the same species exhibit a large degree of variation in fruit quality traits, which can be directly influenced by differences in gene expression due to allelic variations and interactions with the environment. For Malus× domestica Borkh. (apple), fruit quality traits, including color, texture, aroma, flavor profile, and shelf life, are of utmost economic importance. In order to ide...

CONTEXT The identification of genes involved in tumorigenesis is essential for the development of new treatment strategies or diagnostic approaches for pancreatic cancer. OBJECTIVE To identify genes overexpressed in pancreatic cancer we employed differential display, a PCR-based method of differential expression cloning. Using this method, we identified a PCR product that was consistently ove...

Testicular germ cell tumors are important neoplasms and seminoma accounts for 40 to 50% of these tumors. Little is known concerning the molecular events underlying the development of these malignancies. In the present study we used a modified differential display approach to compare gene expression between seminoma and normal testicular parenchyma, both of which are mixed tissues. We first anal...

Screening for differentially expressed genes is a straightforward approach to study the molecular basis of a biological system. In the last 10 years, differential screening technology has evolved rapidly and currently high-throughput tools for genome-wide transcript profiling, such as expressed sequence tags and microarray analysis, are becoming widely available. Here, an overview of this (r)ev...

MOTIVATION Polymerase chain reaction (PCR)-based RNA fingerprinting is a powerful tool for the isolation of differentially expressed genes in studies of neoplasia, differentiation or development. Arbitrarily primed RNA fingerprinting is capable of targeting coding regions of genes, as opposed to differential display techniques, which target 3' non-coding cDNA. In order to be of general use and ...

Differential display is a breakthrough in gene isolation methods (1,2). Compared with other gene isolation methods, it is powerful and relatively rapid. The method achieves isolation of differentially expressed genes by using four 3′-end degenerate primers and 20–40 5′-end arbitrary primers to perform RT–PCR. The PCR products are separated on a DNA sequencing gel and the differences between two...

This research project was intended to analyze the differences in gene expression at different life stages of drosophila melanogaster by using a differential display approach. I focused on the adult and pupae stages. The gene expression of the larvae and egg stages were not analyzed because the larvae and eggs were too small to collect. First I isolated RNA from two different generations of adul...

In the literature, Improved Recycling Folded Cascode (IRFC) Operational Transconductance Amplifier (OTA) is proposed for enhancing the DC gain and the Unity Gain Bandwidth (UGB) of the Recycling Folded Cascode (RFC) OTA. In this paper, an enhanced IRFC (EIRFC) OTA which uses positive feedback at the cascode node is proposed for enhancing the differential mode (DM) gain without changing the unit...