Granulocyte alkaline phosphatase (ALP) activity is measured kinetically using the GEMSAEC centrifugal analyzer and p-nitrophenylphosphate as substrate. Measurements of enzyme activity are made at pH 9.8 utilizing 1.0 M diethanolamine as a buffer containing 0.5 mM magnesium chloride. Granulocytes are separated from heparinized blood using dextran sedimentation, followed with ficoll-hypaque separ...

The biodegradability of four novel diethanolamine derivative complexing agents was examined by using two biodegradation tests standardised by OECD (301B and 301F). Ethylenediaminetetraacetic acid (EDTA) and diethylenetriaminepentaacetic acid (DTPA) were employed as reference substances. Biodegradation of the new complexing agents was studied both with unacclimated and acclimated inocula as well...

This work presents an optimized design of a methyl diethanolamine CO 2 capture unit for clean hydrogen production via steam methane reforming natural gas, where is captured from the pressurized water-gas shifted syngas. We propose modified version standard MDEA-based process flowsheet, and we optimize operating conditions by solving multi-objective optimization problem to minimize energy consum...

A test kit (Iso-ALP, Boehringer Mannheim) for measuring human bone alkaline phosphatase activity in serum or plasma was evaluated in five laboratories in three countries. The assay is based on the principle described by Rosalki and Foo (Clin Chem 1984;30:1182-6) and uses wheat germ lectin to precipitate bone alkaline phosphatase. Residual ALP in the supernate in comparison with total ALP is use...

Maleic hydrazide (1, 2-dihydropyridazine-3, 6dione) is an organic plant growth regulator which has found use in such varied areas as prevention of sucker formation in tobacco, prevention of sprouting of some stored vegetables, and as a herbicide. In commercial application the diethanolamine salt (diethanolammonium maleic hydrazide or diethanolammonium-1 ,2-dihydropyridazine-3, 6-dione) is often...

To separate liver and bone alkaline phosphatase (ALP) isoenzymes in human serum, we used high-performance affinity chromatography (HPAC) on a column of wheat-germ lectin conjugated to 7-microns-diameter silica particles and an eluent containing N-acetyl-D-glucosamine (NAG). On-line spectrophotometric detection of ALP involved pumping diethanolamine-buffered p-nitrophenyl phosphate solution post...

In this study, the solubility of CO2 in DEA in the presence and absence of choline chloride was reported at different temperatures of 276.15, 298.15, 313.15, and 333.15 K, and pressure range of 4-15 bar. The solubility of CO2 was evaluated using the pressure decay method in a batch isochoric stirred absorption cell. Also, the design of experiments performed with Qualitek-4 software using the Ta...

The article presents research on the purification process of pyrolysis gas from hydrogen sulphide and CO2 which release waste tyre process. Pyrolysis, a for treating tyres to create priceless oil, coal, products, is gaining popularity, though. Chemically speaking, oil quite complex contains polar fractions as well almon, aromatic, heteroatoms. fuel properties reveal that it similar or light bur...