BACKGROUND Cytokines and chemokines are relevant biomarkers of pathology and immunity to infectious diseases such as malaria. Several commercially available kits based on quantitative suspension array technologies allow the profiling of multiple cytokines and chemokines in small volumes of sample. However, kits are being continuously improved and information on their performance is lacking. M...

Background: Anti-HCV test is currently used in the diagnosis of hepatitis C, while hepatitis C virus ribonucleic acid (HCV RNA) is used in the determination of viral load. Recently developed hepatitis C virus core antigen (HCV Ag) test is also used in the diagnosis of hepatitis C. The aim of this study was to compare HCV Ag and quantitative HCV RNA in terms of sensitivity and specificity as wel...

BACKGROUND Polymerase chain reaction (PCR) assay has widely used for the detection of tuberculosis (TB). This study tried to compare in-house PCR with some well-known commercial PCR kits for detection of TB agent. METHODS Clinical samples obtained from 620 TB suspected patients were analyzed for the diagnosis of Mycobacterium tuberculosis complex (MTC) by in-house PCR. All samples were obtain...

OBJECTIVES To assess the diagnostic performance of 11 commercial PR3- and MPO-ANCA ELISA systems (direct, capture and high sensitive [hs] ELISA). METHODS Sera from 90 patients with AAV (GPA, MPA and CSS) and 20 disease controls (SLE; RA) and healthy individuals were tested for the presence of ANCA by IFT and by different ELISAs for the presence of PR3-and MPO-ANCA, respectively. Furthermore, ...

The emergence of exploit kits is one of the most important developments in modern cybercrime. Much of cybersecurity research in the recent years has been devoted towards defending citizens from harm delivered through exploit kits. In this paper, we examine an alternate, counter-offensive strategy towards combating cybercrime launched through exploit kits. Towards this goal, we survey a wide ran...

One-step reverse-transcription quantitative polymerase chain reaction (qRT-PCR) is the most widely applied method for COVID-19 diagnostics. Notwithstanding facts that one-step qRT-PCR well suited diagnosis of and there are many commercially available kits in market, their high cost unavailability due to airport closures shipment restriction became a major bottleneck had driven desire produce ke...

Introduction: The introduction of P. falciparum encoded HRP-2 based malaria Rapid Diagnostic Test (RDT) kits is widely accepted in Nigeria and worldwide as a simplified form diagnosis cheaper alternative to the microscopy technique (gold standard). However, deletion Pfhrp2 gene contributes false negative results large number such deletions has been reported advanced countries thereby highlighti...

Peste des petits ruminants (PPR) which is caused by small ruminant morbillivirus (PPRV) has an important economic impact on farming due to high mortality rates, weight loss and restrictions the export of products. Molecular assays are commonly used in diagnosis disease. Extraction RNA from formalin-fixed paraffin-embedded (FFPE) tissues challenging because often degraded formalin fixation proce...

Many investigators who do high-throughput sequencing of mRNA (mRNA-Seq) use kits for library preparation purchased from Illumina. Recognizing that these kits are continually being updated and improved, we provide here some background information, tips, and troubleshooting advice for the kits available at the time of this writing.

Three licensed and three research human T-cell lymphotropic virus type I (HTLV-I) enzyme immunoassay (EIA) kits were evaluated with a panel of 213 serum or plasma samples which were previously tested by the indirect immunofluorescence method for HTLV-I and -II antibodies. The three research kits were found to be more sensitive and specific than the three licensed kits.