Cultured human skin fibroblasts incubated at 37 degrees C with sonically dispersed, positively charged liposomes containing sphingomyelin internalized and metabolized the phospholipid. Sphingomyelin incorporation into the cells produced a reduction in low density lipoprotein binding and degradation. Lecithin-containing liposomes were much less effective. In addition, incubation with sphingomyel...

OBJECTIVE There may be an association between a low serum cholesterol level and dissociative disorders. METHOD The subjects of the study were 16 patients with dissociative disorder and 16 normal comparison subjects (two men and 14 women in each group). Total cholesterol, triglyceride, high-density lipoprotein, low-density lipoprotein, and very low density lipoprotein levels were compared. R...

In order to provide uniqe insight into the metabolic disturbences seen after calving cholesterol, triglycerid, high density lipoprotein, low density lipoprotein, very low density lipoprotein, glucose and insulin levels in serum were studied before calving (group I), in aerly (group II) and late (group III) lactation in 24 normal cows. Serum lipoproteins were separeted into various density class...

1. The lipid compositions of the low-density lipoproteins, the high-density lipoproteins and the ultracentrifugal residue of human serum are presented, with emphasis on certain lipoprotein classes and lipid components not previously described. 2. Except for the lipoproteins with the lowest and highest densities, there is a trend for stepwise successive increase or, respectively, decrease in the...

objective: dyslipidemia and insulin resistance (ir), occurring in most infertile women with polycystic ovarian syndrome (pcos), increase the risk of cardiovascular disease (cvd) and type 2 diabetes. this study aimed to assess the relationships between lipoprotein ratios and ir in pcos women. materials and methods: thirty six infertile women with pcos selected based on androgen excess society (a...

The lipoprotein secreted by cultured eel hepatocytes was fractionated by density gradient ultracentrifugation and compared with eel serum lipoproteins. Eel hepatocytes were cultured for 7 to 10 days as a monolayer in Williams' medium E containing 5% fetal bovine serum and 0.16 microM insulin on a dish precoated with fibronectin of horse serum. The only lipoprotein secreted by eel hepatocytes wa...

The density distribution of lipoproteins in rats fed chow or chow containing 1% cholesterol and 10% olive oil was studied. Lipoprotein fractions were prepared in the ultra-centrifuge between narrow density bands within the density range of 1.006-1.21 and were analyzed by chemical, electrophoretic, and immunological methods. In serum from normal rats there were three major lipoprotein fractions,...

1. A simple technique of 'rosette' formation by low-density-lipoprotein-coated erythrocytes around human lymphoblastoid cells, stimulated to generate specific high affinity low-density lipoprotein receptors, is described. 2. Rosette formation is specific for high-affinity surface binding alone and the properties of the low-density lipoprotein receptors defined by it are identical with those fou...

Modification of low density lipoproteins by human arterial smooth muscle cells was characterized by increased electrophoretic mobility and increased content of malondialdehyde-like oxidation products reactive with thiobarbituric acid. Lipoprotein modification was promoted by micromolar concentrations of iron or copper in the culture medium and was metal ion concentration- and time-dependent. Th...