There is an increase in the number of human infections associated with yeast species that are also known to be involved processing foods such as Saccharomyces cerevisiae, Debaryomyces hansenii, Wickerhamomyces anomalus, or Kluyveromyces marxianus. Clinical strains S. cerevisiae have been well studied over years and much regarding their virulence. However, very little about virulent potential D....

The catabolism of fatty acids is important in the lifestyle of many fungi, including plant and animal pathogens. This has been investigated in Aspergillus nidulans, which can grow on acetate and fatty acids as sources of carbon, resulting in the production of acetyl coenzyme A (CoA). Acetyl-CoA is metabolized via the glyoxalate bypass, located in peroxisomes, enabling gluconeogenesis. Acetate i...

Debaryomyces hansenii is traditionally described as a halotolerant non-conventional yeast and has served model organism for the study of osmotolerance salt tolerance mechanisms in eukaryotic systems past 30 years. However, unraveling D. hansenii's biotechnological potential always been difficult due to persistent limitations availability efficient molecular tools this yeast. Additionally, there...

Bu çalışmada, Düzce ilinden toplanan Lamiaceae familyasına ait Teucrium chamaedrys L. subsp. bitkisinden elde edilen etanol, etil asetat ve kloroform ekstraktları Escherichia coli, Streptococcus pyogenes, Proteus vulgaris, Klebsiella pneumoniae, Staphylococcus aureus, Pseudomonas aeruginosa, Enterococcus faecalis, Salmonella typhimurium, Bacillus cereus subtilis gibi bakteri kültürlerine Candid...

Durum wheat grain can accumulate mycotoxins because it is highly sensitive to infections caused by pathogens of the genera Fusarium and Alternaria. Reduced fungicide use increases demand for biological methods pathogen control. The aim experiment was evaluate efficacy Debaryomyces hansenii (Dh) yeast in reducing content secondary fungal metabolites present spikes five durum cultivars grown sout...

The oleaginous yeast Debaryomyces hansenii is a good model to understand molecular mechanisms involved in halotolerance because of its impressive ability to survive under a wide range of salt concentrations. Several cellular adaptations are implicated in this response, including the presence of a cyanide-insensitive ubiquinol oxidase (Aox). This protein, which is present in several taxonomical ...

Short-chain dehydrogenase reductases (SDRs) have been utilized for catalyzing the reduction of many aromatic/aliphatic prochiral ketones to their respective alcohols. However, there is a paucity of data that elucidates their innate biological role and diverse substrate space. In this study, we executed an in-depth biochemical characterization and substrate space mapping (with 278 prochiral keto...

Binding of substrates into the active site, often through complementarity of shapes and charges, is central to the specificity of an enzyme. In many cases, substrate binding induces conformational changes in the active site, promoting specific interactions between them. In contrast, non-substrates either fail to bind or do not induce the requisite conformational changes upon binding and thus no...