A high-throughput TaqMan PCR assay for detection of bovine paratuberculosis was evaluated by using fecal samples from 1,808 dairy cattle in seven naturally infected herds and 347 dairy cattle in seven herds considered free of paratuberculosis. Fecal, blood, and milk samples were submitted to laboratories where the PCR-based assay, three different fecal culture procedures for Mycobacterium avium...

Background: The Bovine Viral Diarrhea Virus (BVDV) infection causes reproductive and respiratory disease in cattle and buffaloes and is an economically important livestock disease across the world. In Nepal, cattle and buffalo farming is an important source of income for a majority of farmers. Infectious reproductive and respiratory diseases are common in Nepalese dairy herds and a large propor...

Introduction Kenya has one of the most successful dairy industries in sub-Saharan Africa based on some 3 million cattle of mostly high grade Bos taurus dairy breeds and a relatively advanced milk marketing infrastructure. The main breeds for dairy production are Friesian, Guernsey, Ayrshire and their crosses with the local East African Zebu (EAZ). The EAZ population is estimated at about 10 mil...

Three-hundred and eighty-five serum samples were taken from dairy cows on 90 farms in 12 counties from the northern region of the Paraná State, Brazil. The samples were analyzed by IFAT for the detection of anti-Neospora caninum and anti-Toxoplasma gondii IgG antibodies. Forty-five (12%) samples were seropositive to N. caninum, while 102 (26%) samples were seropositive to T. gondii. Only four a...

The importance of cats in the Leptospira epidemiology is due to the possibility of transferring leptospirosis to wild and domesticated animals. The purpose of this survey was to determine the prevalence of Leptospira infection in shorthair cats in different location of Mashhad, Iran. Totally, 147 blood samples were taken from 42 (28.57%), 52 (35.37%) and 53 (36.05%) households...

Mycobacterium avium subsp. paratuberculosis (Map) causes Johne's disease, with large economic consequences for dairy cattle producers worldwide. Map spread between farms is mainly due to animal movements. Locally, herd size and management are expected to influence infection dynamics. To provide a better understanding of Map spread between dairy cattle farms at a regional scale, we describe the ...

OBJECTIVE To describe antimicrobial susceptibility patterns in Campylobacter spp isolated from dairy cattle and farms managed organically and conventionally in the midwestern and northeastern United States. DESIGN Longitudinal study. SAMPLE POPULATION 128 farms. PROCEDURE Samples and data were collected every 2 months from August 2000 to October 2001. Fecal samples were collected from cal...

Campylobacter are important human foodborne pathogens known to colonize the gastrointestinal tract of cattle. The incidence of Campylobacter in cattle may be seasonal and may vary among age groups and type (beef versus dairy). Less is known about other factors that could influence the prevalence, colonization site, and shedding of Campylobacter in cattle. The objectives of this study were to ev...

Farm management practices that reduce the prevalence of food-borne pathogens in live animals are predicted to enhance food safety. To ascertain the potential role of livestock bedding in the ecology and epidemiology of Escherichia coli O157:H7 on farms, the survival of this pathogen in used-sand and used-sawdust dairy cow bedding was determined. Additionally, a longitudinal study of mature dair...

the importance of cats in the leptospira epidemiology is due to the possibility of transferring leptospirosis to wild and domesticated animals. the purpose of this survey was to determine the prevalence of leptospira infection in shorthair cats in different location of mashhad, iran. totally, 147 blood samples were taken from 42 (28.57%), 52 (35.37%) and 53 (36.05%) households, stray and cats w...