The cooperativity of oxygen binding by different subunits in human hemoglobin is greatly reduced by partial deuteration. The effect depends on the time of exposure: n in Hill’s equation decreases from 2.8 to about 1.9 after the hemoglobin has been in D20 for 20 hours at 4”. Further incubation results in a gradual return to normal cooperativity: n E? 2.8 after 240 hours. These effects could be e...

The differences between the boiling points of dilute aqueous solutions of heavy water and the boiling point of ordinary water were measured in the range from 0.3 to 7 percent of D 20. The boiling points were found to increase linearly with increase in D 20, according to the equation mole % of D20 = 70.9 (tJ.T), where tJ.T is the boiling point in degrees centigrade minus 100. The measmements, ma...

of propositions This concept revolves around organizations and the differences of their embeddedness in the macro patterns of routines (economic policy regimes), which in turn may differentially provide them – and the system as a whole – with ‘procedural rationality’ in dealing with identified problems in their relevant complex environment. Regularities of interdependence are specified between ...

The first application of polarization-sensitive multiplex coherent antiStokes Raman spectroscopy (MCARS) in the absence of resonance enhancement to the resolution of the secondary structure of a protein in solution is reported. Polarization MCARS spectra of bovine albumin in D20 were obtained in the range 1370 to 1730 cm -I with the aid of the background suppression technique. The spectra were ...

B. subtilis tryptophan tRNA was isolated utilizing a dot blot hybridization method (1) and its RNA sequence including a modified nucleotide was determined by 2D TLC (2, 3) and UV spectroscopy (4). As a result, we identified five modified nucleotides, (ribothymidine (T54), pseudouridine (¥55, *31), M-isopentenyladenosine (iA37) and dihydrouridine (D20)). In B.subtUis, it has been reported that a...

On the assumption that the double-coiled pattern of microtubules in the axoneme of Echinosphaerium might be due to links of two sizes between adjacent microtubules, we disassembled microtubules with low temperature and then carefully analyzed the patterns of microtubules that formed upon the addition of heat (22 °C) or heat and D20. Although most of the initial clusters of microtubules that for...

Introduction: The paper addresses the effect of a composite modifying mineral additive based on waste — tailings from Balkhash Mining and Processing Plant microsilica structural characteristics performance heavy concrete. Purpose study: We aimed to select optimal B25 B35 concrete mixes MV-D20 modified binder with in its composition evaluate Methods: In course study, standard methods were used d...