We have expressed the human isoforms of the liver-type (GLUT2) and brain-type (GLUT3) facilitative glucose transporters in oocytes from Xenopus laevis via injection of in vitro transcribed mRNA. As reported previously [Gould, Thomas, Jess and Bell (1991) Biochemistry 30, 5139-5145], GLUT2 mediates the transport of fructose and galactose, and GLUT3 mediates the transport of galactose. We have ex...

Untransformed as well as polyoma virus-transformed hamster cells can be grown equally well on a slow catabolite like galactose as on a rapid catabolite like glucose. The rate of uptake of galactose is greatly enhanced in the transformed cells as compared with untransformed cells, and this enhancement of entry was as markedly expressed in galactose-grown cultures as in glucose-grown cultures. Si...

The metabolic pathway of 2-deoxy-2-fluoro-D-galactose (FDGal) in mice was studied by 19F NMR. Efficient accumulation of FDGal in liver was demonstrated by NMR, which is consistent with the results of Ishiwata et al. using radioactive 18FDGal. The new discovery is that this fluorinated hexose was converted to 2-deoxy-2-fluoro-D-glucose (FDG) through UDP-FDGal and UDP-FDG apparently by the action...

The alkali-catalyzed oxidative degradation of lactose (1) to potassium O-beta-D-galactopyranosyl-(1----3)-D-arabinonate (2) has been studied and compared with that of D-glucose to D-arabinonate and D-galactose to D-lyxonate. A mechanism for the degradation of 1 catalyzed by alkali only is presented and discussed, taking into consideration the main reaction products. Increasing the reaction temp...

Background: Aging in the male reproductive system can cause testicular degeneration. Nowadays, regenerative medicine is a developing domain. This study conducted to describe potential effects of dried bovine placenta supplementation on percentage abnormal spermatozoa, density seminiferous tubules luminal content and Leydig cells counts D-galactose-induced aging rats. Methods: A total 15 healthy...

In 1931 Wierzuchowski (1) showed an influence of insulin on galactose tolerance of dogs. More recently, Levine, Goldstein, Huddlestun, Klein, and Henry (2, 3) demonstrated that insulin lowers the blood levels of D-galactose, D-xylose, and L-arabinose but not of D-arabinose or D-mannose, following their infusion into the eviscerated nephrectomized dog. The volume of distribution of the responsiv...