the thermodynamic functions such as enthalpy (h), gibbs free energy (g) and entropy (s) of serineand threonine amino acids were theoretically studied at different condition (solvents andtemperatures) by using gussian o3, software. first, the structural optimization of isolated serine andthreonine were done in the gas phase by using the hartree-fock (hf) level of theory with 3-21g, 6-31g and 6-3...

The digestibility of amino acids in sow's milk consumed by young pigs is currently unknown because of difficulties associated with collecting an adequate quantity of milk, and also problems in cannulating suckling pigs. A total of 14 kg of sow's milk was collected, two soluble indigestible markers (Co-EDTA and YbC13) were added, and the milk was fed to four pigs at 17 d of age that were fitted ...

The impact of PGF2? on total proteins, free amino acids and the amino transferases in the gonads of silkworm, Bombyx mori L. were investigated in the present experiment. The effect of PGF2? resulted in increase in the total protein and free amino acid contents in the gonads of silkworm larvae indicating improved structural and dynamic levels of organization. The enhanced activity levels of amin...

It was found that D-stereoisomers of natural amino acids inhibit the growth of Saccharomyces cerevisiae cells. Kinetic and genetic evidence showed that d-amino acids enter the cell by the general amino acid permease. Two classes of S. cerevisiae mutants resistant to d-amino acids were isolated. One class of mutants appeared to be defective in the general amino acid permease specified by the gen...

We describe a simple method for enzymatic synthesis of L and D amino acids from a-keto acids with Escherichia coli cells which express heterologous genes. L-amino acids were produced with thermostable L-amino acid dehydrogenase and formate dehydrogenase (FDH) from a-keto acids and ammonium formate with only an intracellular pool of NAD for the regeneration of NADH. We constructed plasmids conta...

We describe a simple method for enzymatic synthesis of L and D amino acids from alpha-keto acids with Escherichia coli cells which express heterologous genes. L-amino acids were produced with thermostable L-amino acid dehydrogenase and formate dehydrogenase (FDH) from alpha-keto acids and ammonium formate with only an intracellular pool of NAD+ for the regeneration of NADH. We constructed plasm...

Amino acids having a dextro configuration have been found in natural material from two sources.’ Jacobs and Craig obtained d-proline from ergotinine (4) and later other ergot alkaloids, and IvAnovics and Bruckner found d( -)-glutamic acid as the principal constituent of the P-antigen of Bacillus anthracis and other microorganisms of the related Bacillus mesentericus-subtilis group (5). Inasmuch...

In a previous paper (1) evidence was presented that acid hydrolysates of the cellular material of BaciZZus brevis (ATCC 10068) contained an unusually high content of amino acids of the D configuration, amounting to over 10 per cent of the total amino acids or 5 per cent of the dry weight of the cells. The n-amino acids were shown to exist in the intact cells, and not to result from racemization...

introduction: radioimmunotherapy (rit) is a very promising new therapy for the treatment of recurrent b-cell non-hodgkin's lymphoma (nhl). iodine-131 is the most frequently used nuclide in clinical rit, but its usefulness has been limited by dehalogenation of monoclonal antibodies labeled via conventional methods. to circumvent this problem, we have synthesized a tri-peptide consisting of non-m...

A bacterial translation factor EF-P alleviates ribosomal stalling caused by polyproline sequence by accelerating Pro-Pro formation. EF-P recognizes a specific D-arm motif found in tRNAPro isoacceptors, 9-nt D-loop closed by a stable D-stem sequence, for Pro-selective peptidyl-transfer acceleration. It is also known that the T-stem sequence on aminoacyl-tRNAs modulates strength of the interactio...