The aims of this study are to determine the virulence factors parasitic infestation, especially E.histolytic parasite, for persons with cancer and parasites. This contain 2 groups: Group (G1) Peoplewith diarrhea due parasite (50)and (G2) People parasites (24), Stoolsamples were collected from both groups purpose confirming diagnosis microscopicallydiagnosed parasite,Molecular testing is used co...

Entamoeba histolytica, likes other Organismes, is characterized by diversity and heterogeneity in its genetic content, which one of the most paramount reasons for survival, increase susceptibility to infection. Non-condensation chromosomes during process cell division ambiguity chromosomal ploidy makes predicting exact numeral difficult. Genes distributed across 14 as well many extra-chromosome...

The main protease (Mpro) of SARS-CoV-2 is a current target for the inhibition viral replication. Through combined Docking and Density Functional Theory (DFT) approach, we investigated in-silico molecular mechanism by which ebselen (IUPAC: 2-phenyl-1,2-benzoselenazol-3-one), most famous pharmacologically active organoselenide, inhibits Mpro. For first time, report on mechanistic investigation in...

Proteolytic activity of 0-12 day old eggs, miracidium and adult worm of Fasciola gigantica was assessed and proteases were partially purified by DEAE-Sepharose and CM-cellulose columns. Four forms of protease were separated, PIa, PIb, PIc and PII. Purifications were completed for PIc and PII using Sephacryl S-200 chromatography. A number of natural and synthetic proteins were tested as substrat...

Since protease activity is highly regulated by structural and environmental influences, the abundance of a protease often does not directly correlate with its activity. Because in most of the cases it is the activity of a protease that gives rise to its biological relevance, tools to report on this activity are of great value to the research community. Activity-based probes (ABPs) are small mol...

Treatment of U937 human monocyte-like cells with Streptococcus pyogenes led to an induction of apoptosis in these cells. A comparison between the wild-type strain and its isogenic protease-negative mutant indicated that the production of streptococcal pyrogenic exotoxin B (SPE B), a cysteine protease, caused a greater extent of apoptosis in U937 cells. Further study using purified SPE B showed ...

Raphides, needle-shaped calcium oxalate crystals in tissues of many plants, have been thought to play defensive roles against herbivores without detailed bioassays for their defensive roles and modes of function using purified raphides. In order to examine the defensive roles and modes of function of raphides in a clear experimental system, we performed bioassays giving the larvae of the Eri si...

BACKGROUND Trypanosoma cruzi has a single flagellum attached to the cell body by a network of specialized cytoskeletal and membranous connections called the flagellum attachment zone. Previously, we isolated a DNA fragment (clone H49) which encodes tandemly arranged repeats of 68 amino acids associated with a high molecular weight cytoskeletal protein. In the current study, the genomic complexi...

The N terminus of the replicase nonstructural protein 2 (nsp2) of porcine reproductive and respiratory syndrome virus (PRRSV) contains a putative cysteine protease domain (PL2). Previously, we demonstrated that deletion of either the PL2 core domain (amino acids [aa] 47 to 180) or the immediate downstream region (aa 181 to 323) is lethal to the virus. In this study, the PL2 domain was found to ...

Since the discovery that Der p 1 is a cysteine protease, the role of proteolytic activity in allergic sensitization has been explored. There are many allergens with proteolytic activity; however, exposure from dust mites is not limited to allergens. In this paper, genomic, transcriptomic and proteomic data on Dermatophagoides pteronyssinus (DP) was mined for information regarding the complete d...