Mitochondrial homoplasmy signifies the existence of identical copies of mitochondrial DNA (mtDNA) and is essential for normal development, as heteroplasmy causes abnormal development and diseases in human. Homoplasmy in many organisms is ensured by maternal mtDNA inheritance through either absence of paternal mtDNA delivery or early elimination of paternal mtDNA. However, whether paternal mtDNA...

Carp (Cyprinus carpio) red cells do not show beta-adrenergic responses when incubated with 10(-5) mol l-1 adrenaline at atmospheric oxygen tension and a pH value close to the in vivo resting pH (approx. 8.1). However, when either the pH or the oxygen tension of the incubation medium is decreased, the adrenergic responses appear, showing that oxygen or an oxygen-linked phenomenon has a direct in...

Generalist fish species can feed on a wide resource spectrum and across trophic levels depending availability interactions. Crucian carp (Carassius carassius) represents good candidate to investigate variation in the ecology of generalist as it be found highly variable communities its use is well documented. In this study, we explored crucian at individual population using stable isotope gut co...

While a large respiratory surface area is good for gas exchange, it also poses several problems, including energetically unfavorable fluxes of water and ions. As a result, fishes appear to have a respiratory surface area that is matched to their oxygen demands. When faced with changes in their need for oxygen uptake, e.g. through altered physical activity or altered ambient oxygen levels, fishe...

Herein we demonstrated the effect of oxygen and air atmosphere on the success of short-term storage of sperm obtained from rainbow trout (Oncorhynchus mykiss), sterlet (Acipenser ruthenus) and common carp (Cyprinus carpio) diluted in immobilizing media (100 mM NaCl, 40 mM KCl, 3 mM CaCl2, 1.5 mM MgCl2, 50mM Tris, pH 8.2 for salmonid fish, 17 mM NaCl, 1.7 mM KCl, 6 mM NaHCO3, 0.5 mM CaCl2, 0.5 m...

The metabolic-level boundaries (MLB) hypothesis and the cell metabolism (CM) hypothesis have been proposed to explain the body mass scaling of metabolic rate. The MLB hypothesis focuses mainly on the influence of the metabolic level on the relative importance of volume and surface area constraints. The CM hypothesis focuses on the variation of cell size as the body grows. The surface area to vo...

Furunculosis is a serious, septicemic, bacterial disease found principally in salmonid fishes. but it may also occur in goldfish and other cyprinids. The common name of the disease is derived from the presence of “blisters” or furuncules on the surface of chronically infected salmonids (Snieszko and Bullock 1975). However, this sign is not diagnostic of this disease inasmuch as it may be encoun...

Carassius auratus var. pingxiangnensis is a natural triploid crucian carp mutant. In order to understand its placement and genetic background at the gene level, the characteristics of mitochondrial DNA sequences and phylogenetic relationship were examined. The results showed that the mitochondrial DNA is a circular double-stranded DNA molecule that is 16,576 bp in length with 13 protein-coding ...

In this study, an enzyme-linked immunosorbent assay (ELISA) was developed to quantify vitellogenin (Vtg) in zebrafish (Danio rerio). Zebrafish Vtg (zf-Vtg) was purified from whole-body homogenates of estradiol-exposed zebrafish, and polyclonal antibodies against zf-Vtg were raised. Using purified zf-Vtg as a standard and anti-zf-Vtg antibodies (DR-264), a competitive ELISA method was set up and...

The present study aimed to identify differentially expressed genes (DEGs) and major signal transduction pathways that were related to the immune response of epithelioma papulosum cyprinid (EPC) cells to reoviruses isolated from allogynogenetic silver crucian carp. The study also lays a theoretical foundation for the pathogenesis and immunity of the reovirus, which is helpful to the breeding of ...