Ethylene plays an important role in wide-ranging aspects of plant growth and development, includingfruit ripening, leaf and flower senescence. In this study, the expression patterns of two genes involved in theethylene signal transduction pathway (RhCTR1 and RhCTR2) were investigated during the flower openingstages in two Rosa hybrida cultivars, ‘Black magic’ and ‘Maroussia’, ...

In situ PCR is a technique that allows specific nucleic acid sequences to be detected in individual cells and tissues. In situ PCR and IS-RT-PCR are elegant techniques that can increase both sensitivity and throughput, but they are, at best, only semiquantitative; therefore, it is desirable first to ascertain the expression pattern by conventional means to establish the suitable conditions for ...

Aquaporins are water channel proteins that facilitate the passage of water through biological membranes and play a crucial role in plant growth. We showed that ethylene treatment significantly reduced petal size, inhibited expansion of petal abaxial subepidermal cells, and decreased petal water content in rose (Rosa hybrida 'Samantha'). Here, we report the isolation of a plasma membrane aquapor...

Rose plants (Rosa x hybrida L. CV. Kardinal, grafted on Natal Brier) were grown in UC mix and in coconut coir. Water release curves and hydraulic conductivity of the media were measured. Water tensions in the media were maintained within a predetermined narrow range by tension-actuated irrigation, using high-flow electronic tensiometers. Transpiration rate of plants sitting on load cells was me...

Dog rose (Rosa canina) has been using as a rootstock for ornamental roses also it is one of the medicinal plants. It can be propagated under in vitro conditions. After removing chilling requirement of buds, axillary wood buds of dog rose were cut in three nodal positions (lower, middle and terminal) on the stem and then explants after decontamination, cultured on Murashige and Skoog medium (MS ...

We have initiated immunological and drug-binding studies on the tubulins from different higher plant species. Antibodies were raised against electrophoretically separated rose (Rosa sp.) tubulin alpha- and beta-subunits and characterized by immunoblot autoradiographic assays. Each IgG preparation bound to its antigen and cross-reacted differentially with the respective tubulin subunits from an ...