We report a method of metabolomic profiling of intact tissue based on molecular preservation by extraction and fixation (mPREF) and high-performance chemical isotope labeling (CIL) liquid chromatography mass spectrometry (LC-MS). mPREF extracts metabolites by aqueous methanol from tissue biopsies without altering tissue architecture and thus conventional histology can be performed on the same t...

XRF 030723 is the first X-ray flash (XRF) to show in its optical light curve (LC) a bump that has been interpreted as the signature of a supernova (SN). After subtracting the afterglow component from the observed optical LC of the XRF counterpart, the properties of the SN are constrained by means of synthetic LCs of core-collapse SNe. For the range of redshift suggested for XRF 030723 (z ∼ 0.3 ...

A sensitive and simple liquid chromatography/tandem mass spectrometry (LC-MS/MS) method was developed for the detection of alginate oligosaccharides (AOs) in mouse plasma and urine after oral administration. In an AO mixture, dimer, trimer, and tetramer were detected by LC-MS/MS equipped with an anion-exchange column with extremely high sensitivity. By this method, we detected certain levels of...

Background: Aberrant lipid metabolism is closely associated with cancer. Materials & Methods: Serum levels of sphingomyelins (SM) (34:1), phosphatidylcholine (PC) (34:2), PC(34:1), PC(36:4), PC(36:3), and PC(36:2) in 1449 serum samples (including 599 normal controls, 69 patients with benign lung diseases (BLDs), 61 with benign colorectal diseases, 54 with benign gastric diseases, 67 with benign...

Late diagnosis of hepatocarcinoma (HCC) is one of the most primary factors for the poor survival of patients. Thereby, identification of sensitive and specific biomarkers for HCC early diagnosis is of great importance in biological medicine to date. In the present study, serum metabolites of the HCC patients and healthy controls were investigated using the improved liquid chromatography-mass sp...

Salidroside and its aglycone p-tyrosol are two major phenols in the genus Rhodiola and have been confirmed to possess various pharmacological properties. In our present study, p-tyrosol was identified as the deglycosylation metabolite of salidroside after intravenous (i.v.) administration to rats at a dose of 50 mg/kg, but was not detectable after intragastric gavage (i.g.) administration throu...

An overview is provided of recent developments in the use of latent class (LC) and other types of %nite mixture models for classi%cation purposes. Several extensions of existing models are presented. Two basic types of LC models for classi%cation are de%ned: supervised and unsupervised structures. Their most important special cases are presented and illustrated with an empirical example. c © 20...

Along with the creation of new maps, current efforts for improving global land cover (GLC) maps focus on integrating maps by accounting for their relative merits, e.g., agreement amongst maps or map accuracy. Such integration efforts may benefit from the use of multiple GLC reference datasets. Using available reference datasets, this study assesses spatial accuracy of recent GLC maps and compar...

In this month’s “Column Watch,” Ron Majors discusses advances in multidimensional chromatography and comprehensive multidimensional chromatography. These techniques are well suited for the separation of complex samples. Although GC GC is fairly well developed, LC LC is in its infancy. But with fast LC columns, based on monoliths, superficially porous particles, and short sub-2m particle columns...

OBJECTIVE In our previous study, we found that some miRNAs were deregulated in hepatocellular carcinoma (HCC), including miR-183. However, the expression of miR-183 in the progression of benign liver diseases to HCC and its correlation with clinicopathologic factors remain undefined. METHODS MiR-183 expression was measured in normal controls (NC) (n = 21), chronic viral hepatitis B or C (CH) ...