We investigated the appropriate growth conditions of shoot and root through in vitro propagation of orchid (Dendrobium sonia) using different gelling agents. Protocorm Like Bodies (PLBs) of orchid were initiated earlier in vitro and one month old protocorms were used for this study. Protocorms were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of starche...

Leaf explants of kaladium dwubarwne Caladium bicolor (Aiton) Vent., were cultured in vitro on MS media supplemented with 25 different concentrations of BAP and NAA in order to determine the appropriate concentrations for micropropagation. All combinations induced callus formation on explants. Callus production on leaves explants grown on control medium was very low. The medium enriched with 4 m...

Shoot tips and nodal segment explants to Rauwolfia serpentina when cultured on MS medium containing varying concentration of Benzyl adenine purine (BAP) and Benzyl adenine purine in combination with Indole butyric acid (IBA) produced multiple shoots. Maximum multiple shoots (85.6%) were found in static MS medium supplemented with 5.0 mg/ 1 BAP and 0.5 mg / 1 IBA along with 2.5% sucrose and 0.85...

background: although lowering of food cholesterol is an effective way to reduce blood cholesterol, but recently some effective health and bioactivity characteristics of the milk fat components have been identified, so by decreasing the use of milk fat, an important source of fat-soluble vitamins and bioactive components of milk is limited. recent studies have shown that the growth of lactic aci...

the green colonial microalga, botryococcus braunii is well known for its high lipid content and has already been proposed as a renewable energy source for various aquaculture and biotechnological applications. however, due to its slow growth rate compared with other microalgae, b. braunii has not yet been used in mass culture to produce more biomass. therefore, in this study we tested different...

an in vitro plant regeneration protocol was successfully established in aphyllorchis montana , a saprophytic achlorophyllous orchid by culturing immature seeds. among the six basal media evaluated for seed germination, bm-tm medium was found to be the best followed by kc medium. after 40 days, all the media turned brown and the growths of the protocorms were arrested. activated charcoal, 1 g/l ...

the first successful and an efficient regeneration system on in vitro direct plant regeneration of agastache foeniculum was reported. the experiment were done in factorial design with three replications and explants were cultured in ms media fortified with different concentrations of bap and tdz (0, 2.2, 4.4, 8.8 μm) in combination with 3 levels of iaa (0, 1.1 and 2.2 μm). anova analysis showed...

Plant growth regulators (PGRs) have an important role in callus induction and organogenesis of plant explants cultured in vitro conditions. Callus has a proper potential for plant regeneration through indirect organogenesis and embryogenesis as well suspension culture, genetic transformation and production of secondary metabolites. In current experiment, leaf explants of Petunia × hybrida F1 we...