1. Cases of flagellate infection of man by Chilomastix mesnili have been reported from nearly every locality in the world. They are fairly abundant in the United States and are often confused with cases of infection by Trichomonas intestinalis. 2. The shape of Chilomastix is pyriform. The body contains the following structures: nucleus, centrosome, primary, secondary, and tertiary blepharoplast...

Study of Sarcina ventriculi (Zymosarcina ventriculi) has been encumbered by the absence of a satisfactory stock culture method. Smit (1930), as well as Milhaud et al. (1956) reported that transfers of the organism at 1or 2-day intervals were necessary to keep viable cultures. In this paper a method is described in which a viable stock culture may be maintained for a period of 2 months. A brief ...

A method for toxigenicity testing of Corynebacterium diphtheriae in tissue cultures was developed. Results were obtained by comparing destruction of the monkey kidney or, preferably, rabbit kidney monolayer by 0.1 ml of the C. diphtheriae culture in Elek's broth containing 20% rabbit serum with the appearance after the addition of 0.2 ml of a mixture of the C. diphtheriae culture and diphtheria...

Ever since Koch first cultivated the tubercle bacillus, methods have been sought to hasten its growth (in vitro). In his original communication Koch’ described a technique of examining cultures of tubercle bacilli grown in coagulated blood serum placed in watch glasses or hollow glass slides. He examined these preparations by low-power magnification and was able to detect growth in one week. Pr...

BACKGROUND Early laboratory detection of Mycobacterium tuberculosis is crucial for controlling tuberculosis. We developed a hydrogel mycobacterial culture method that retains the advantages of both solid and liquid methods in terms of speed, cost, and efficiency. METHODS Mycobacterium bovis bacillus Calmette-Guérin (BCG) suspensions and 200 acid-fast bacilli (AFB)-positive clinical specimens ...

Beginning with Edward Tylor’s (1889) definition of culture as socially “acquired,” I focus in this article on motion as social acquisition and transmission through “artifacts”—both durable (like ceramic pots) and fleeting (like sounds). Motion can be detected by comparison of the artifacts to which people are exposed with those they in turn produce. I examine rates of interaction with artifacts...

A method was devised to test the growth-promoting ability of a broth medium. The "dilute to extinction" method determines the inoculum required to develop heavy turbidity in a broth with overnight incubation. A statistical method using Poisson distribution was used to show that a single Haemophilus cell can develop heavy turbidity in an optimal broth. The dilute to extinction method was used to...

they were washed with wet swabs and suspended in 1 ml. of nutrient broth and inoculated on blood agar media and into mice. All tested slides failed to produce either culture or infection. A similar experiment was performed with the same type of slides from which mercuric chloride was removed. Both the medium and mice failed to show the presence of B. anthracis. Because unstained areas round the...

records of the Middle Ages and the Renaissance is that they enable us to study not the learned culture but the beliefs and views of the common people. Were it not for the fact that so many were hauled before the Inquisitorial courts, we would have had very little evidence about them at all. In this particular case, these sources are of inestimable value because they constitute almost the only s...